Screening of Wild Potatoes Identifies New Sources of Late Blight Resistance
Screening of Wild Potatoes Identifies New Sources of Late Blight Resistance
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DOI：
10.1094/pdis-06-20-1367-re
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发表时间：
2021-02-01
期刊：
影响因子：
4.5
通讯作者：
Halterman, Dennis A.
中科院分区：
文献类型：
--
作者：
Karki, Hari S.;Jansky, Shelly H.;Halterman, Dennis A.
马铃薯晚疫病是世界上最具毁灭性的植物病害之一。大多数种植的马铃薯易受这种疾病的影响。然而，马铃薯的野生近缘种是LB抗性的极好来源。我们在离体叶测定（DLA）中筛选了来自美国马铃薯基因库的72种不同野生马铃薯物种的384份加入物对抗LB病原体致病疫霉。在DLA中使用致病疫霉菌分离株US-23和NL 13316来筛选材料。273份材料全部感病，39份材料全部抗病。在33份材料中发现了抗、感病植株。所有测试的植物表现出部分抗性表型在两个加入，分离的抗性和部分抗性植物在9个加入，分离的部分抗性和感病植物在4个加入，和分离的抗性，部分抗性，和感病的个人在24个加入。我们发现了几种以前从未报道过的对LB有抗性的物种：白茄、白茄。agrimoniifolium、S. chomatophilum，S. ehrenbertenes，S. hypacrarthrum，S. iopetalum，S. palustre，S. piurae，S. morelliforme，S. neocardenasii、新卡氏链霉菌S. trifidum和S.托叶。这些新物种可以提供新的LB抗性来源。进行所选物种的致病疫霉克隆品系特异性筛选以鉴定RB抗性的存在。我们在疣果茄、匍匐茎茄和刺果茄中发现了LB抗性材料。morelliforme对RB克服分离物NL 13316敏感，表明这些物种中存在RB样抗性。
Late blight (LB) of potato is considered one of the most devastating plant diseases in the world. Most cultivated potatoes are susceptible to this disease. However, wild relatives of potatoes are an excellent source of LB resistance. We screened 384 accessions of 72 different wild potato species available from the U.S. Potato GeneBank against the LB pathogen Phytophthora infestans in a detached leaf assay (DLA). P. infestans isolates US-23 and NL13316 were used in the DLA to screen the accessions. Although all plants in 273 accessions were susceptible, all screened plants in 39 accessions were resistant. Resistant and susceptible plants were found in 33 accessions. All tested plants showed a partial resistance phenotype in two accessions, segregation of resistant and partial resistant plants in nine accessions, segregation of partially resistant and susceptible plants in four accessions, and segregation of resistant, partially resistant, and susceptible individuals in 24 accessions. We found several species that were never before reported to be resistant to LB: Solanum albornozii, S. agrimoniifolium, S. chomatophilum, S. ehrenbergii, S. hypacrarthrum, S. iopetalum, S. palustre, S. piurae, S. morelliforme, S. neocardenasii, S. trifidum, and S. stipuloideum. These new species could provide novel sources of LB resistance. P. infestans clonal lineagespecific screening of selected species was conducted to identify the presence of RB resistance. We found LB resistant accessions in Solanum verrucosum, Solanum stoloniferum, and S. morelliforme that were susceptible to the RB overcoming isolate NL13316, indicating the presence of RB-like resistance in these species.