Identification and characterization of a cyanate permease in Escherichia coli K-12.
Identification and characterization of a cyanate permease in Escherichia coli K-12.
复制标题
大肠杆菌 K-12 中氰酸盐通透酶的鉴定和表征。
DOI：
10.1128/jb.171.9.4674-4678.1989
复制
发表时间：
1989
影响因子：
3.2
通讯作者：
Fuchs,JA
中科院分区：
文献类型：
--
作者：
Sung,YC;Fuchs,JA
大肠杆菌含有一种可诱导的酶，氰化酶，它能催化氰酸盐分解成氨和碳酸氢盐。克隆了编码氰酶的基因cynS，发现其位于含有乳糖操纵子的DNA片段上。编码氰化酶的质粒的表征表明，一种功能未知的26千道尔顿（kDa）蛋白质也被氰酸盐诱导（Y-C。宋，D. Parsell，P.M.安德森和J.A. Fuchs，J. Bacteriol. 169：2639-2642，1987）。编码26-kDa蛋白的基因位于cynS及其启动子之间，表明存在cyn操纵子。26 kDa的蛋白质被确定为一个氰酸酯通透酶，运输外源氰酸酯的主动运输。E.大肠杆菌显示含有氰酸盐转运系统，该系统是能量依赖性的并且可被氰酸盐饱和。
Escherichia coli contains an inducible enzyme, cyanase, that catalyzes the decomposition of cyanate into ammonia and bicarbonate. The gene encoding cyanase, cynS, was cloned and found to be on a DNA fragment that contained the lac operon. Characterization of a plasmid encoding cyanase indicated that a 26-kilodalton (kDa) protein of unknown function was also induced by cyanate (Y-C. Sung, D. Parsell, P.M. Anderson, and J.A. Fuchs, J. Bacteriol. 169:2639-2642, 1987). The gene encoding the 26-kDa protein was located between cynS and its promoter, indicating the existence of a cyn operon. The 26-kDa protein was identified as a cyanate permease that transports exogenous cyanate by active transport. E. coli was shown to contain a cyanate transport system that is energy dependent and saturable by cyanate.