Potential ameliorative effects of epigallocatechin-3-gallate against cigarette smoke exposure induced renal and hepatic deficits
Potential ameliorative effects of epigallocatechin-3-gallate against cigarette smoke exposure induced renal and hepatic deficits
复制标题
表没食子儿茶素-3-没食子酸酯对香烟烟雾暴露引起的肾脏和肝脏缺陷的潜在改善作用
DOI：
10.1016/j.ecoenv.2020.110202
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发表时间：
2020-03-15
影响因子：
6.8
通讯作者：
Liu, Min
中科院分区：
文献类型：
--
作者：
Chen, Jinglou;Liu, Jianhua;Liu, Min
香烟烟雾造成的环境污染严重危害着人们的健康。表没食子儿茶素没食子酸酯（epigallocatechin-3-gallate，EGCG）是绿色茶中含量最丰富的儿茶素。在这项研究中，大鼠暴露于CS 90天。通过检测血清肌酐和血尿素氮水平评价肾功能。检测肝功能指标谷丙转氨酶、谷草转氨酶活性。通过检测丙二醛、还原型谷胱甘肽、抗氧化酶（超氧化物歧化酶和谷胱甘肽过氧化物酶）和促炎细胞因子的水平来评估肾脏和肝脏的氧化应激和炎症。通过Masson染色观察胶原沉积，通过检查羟脯氨酸水平，通过测量纤维化相关基因胶原（Col）-1A1和Col-3A1的mRNA水平，以及通过评估促纤维化TGF-β 1途径的活性来评估器官纤维化。此外，肾和肝上皮间质转化（EMT）进行了评价。结果表明，EGCG可明显改善CS诱导的大鼠肾脏和肝脏氧化应激、炎症反应、EMT，并抑制CS诱导的TGF-β 1信号通路的激活。这些结果表明，EGCG可以减轻CS诱导的肾和肝纤维化。
The environmental pollution caused by cigarette smoke (CS) seriously endangers people's health. Epigallocatechin-3-gallate (EGCG) is the most abundant catechin in green tea. In this study, rats were exposed to CS for 90 days. Kidney function was evaluated by detecting the levels of serum creatinine and blood urea nitrogen. Liver function was evaluated by detecting the activities of alanine aminotransferase and aspartate transaminase. The renal and hepatic oxidative stress and inflammation were assessed by detecting the levels of malondialdehyde, reduced glutathione, antioxidant enzymes (superoxide dismutase and glutathione peroxidase) and proinflammatory cytokines. Organ fibrosis was evaluated by observing collagen deposition via masson staining, by examining the hydroxyproline level, by measuring the mRNA levels of fibrosis-associated genes collagen (Col)-1A1 and Col-3A1, as well as by assessing the activity of profibrotic TGF-beta 1 pathway. Additionally, renal and hepatic epithelial-mesenchymal transition (EMT) were evaluated. It was observed that EGCG ameliorated the renal and hepatic oxidative stress, inflammation, EMT, as well as inhibited the activation of TGF-beta 1 signaling pathway induced by CS. These results showed that EGCG could attenuate CS-induced renal and hepatic fibrosis.