Spatial Organization in Proteinaceous Membrane-Stabilized Coacervate Protocells
Spatial Organization in Proteinaceous Membrane-Stabilized Coacervate Protocells
复制标题
蛋白质膜稳定凝聚层原始细胞的空间组织
DOI：
10.1002/smll.201902893
复制
发表时间：
2019-09-04
期刊：
影响因子：
13.3
通讯作者：
Huang, Xin
中科院分区：
文献类型：
--
作者：
Li, Junbo;Liu, Xiaoman;Huang, Xin
作为一种模型原始细胞，无膜凝聚层微滴被广泛用于功能研究，以深入了解细胞的物理化学性质并设计拟细胞软技术;然而，缺乏离散的膜导致其不稳定并限制了进一步的应用。在此，开发了一种策略，以制造基于蛋白质膜在凝聚微滴表面的自组装的混合原始细胞，所述凝聚微滴由静电粘附和空间/亲水表面浮力的组合驱动。半透性蛋白质膜能明显提高凝聚体的稳定性，而不影响其螯合作用。值得注意的是，这样的杂交原细胞展示了空间组织，由此各种功能酶可以位于离散区域中，这促进了级联酶促反应的开/关调节，沿着亚群之间增强的化学通讯。
As a model protocell, the membrane-free coacervate microdroplet is widely utilized in functional studies to provide insights into the physicochemical properties of the cell and to engineer cytomimetic soft technologies; however, the lack of a discrete membrane contributes to its instability and limits further application. Herein, a strategy is developed to fabricate a hybrid protocell based on the self-assembly of a proteinaceous membrane at the surface of coacervate microdroplets driven by a combination of electrostatic adhesion and steric/hydrophilic surface buoyancy. The semipermeable proteinaceous membrane can enhance coacervate stability obviously without compromising sequestration behavior. Significantly, such hybrid protocells demonstrate spatial organization whereby various functional enzymes can be located in discrete regions, which facilitates an on/off modulation for a cascade enzymatic reaction along with enhanced chemical communication between subpopulations.