Chemical Coding for Cardiovascular Sympathetic Preganglionic Neurons in Rats
Chemical Coding for Cardiovascular Sympathetic Preganglionic Neurons in Rats
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DOI：
10.1523/jneurosci.0796-10.2010
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发表时间：
2010-09-01
影响因子：
5.3
通讯作者：
Anderson, Colin R.
中科院分区：
文献类型：
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作者：
Gonsalvez, David G.;Kerman, Ilan A.;Anderson, Colin R.
可卡因和安非他明调节的转录肽(CART)存在于大鼠交感神经节前神经元的一个亚群中。我们观察了CART免疫反应终末在大鼠颈上星状神经节和肾上腺的分布，发现它们环绕神经肽Y免疫反应阳性的节后神经元和去甲肾上腺素能嗜铬细胞。星状神经节和颈上神经节的CART免疫反应阳性节前神经元的靶点是供应肌肉和皮肤的血管收缩神经元和心脏投射的节后神经元：它们不以支配唾液腺、竖起肌、棕色脂肪或肾上腺素能嗜铬细胞的非血管收缩神经元为靶标。伪狂犬病病毒的跨神经元追踪显示，在前肢骨骼肌的血管收缩通路中，许多节前神经元呈CART免疫反应阳性，但不是全部。同样，共聚焦显微镜分析证实，与血管收缩神经节细胞接触的牛顿中有70%含有CART，而30%不含CART。最后，我们发现，在全身缺氧后，CART免疫反应阳性细胞占表达c-Fos的节前神经元总数的69%。我们得出结论，CART存在于大多数(尽管不是全部)心血管节前神经元中，但不存在于具有非心血管靶点的胸段节前神经元。我们认为，CART免疫反应可能识别出假定的“副”节前神经元，其作用可能是放大血管运动性神经节传递。
Cocaine and amphetamine-regulated transcript peptide (CART) is present in a subset of sympathetic preganglionic neurons in the rat. We examined the distribution of CART-immunoreactive terminals in rat stellate and superior cervical ganglia and adrenal gland and found that they surround neuropeptide Y-immunoreactive postganglionic neurons and noradrenergic chromaffin cells. The targets of CART-immunoreactive preganglionic neurons in the stellate and superior cervical ganglia were shown to be vasoconstrictor neurons supplying muscle and skin and cardiac-projecting postganglionic neurons: they did not target non-vasoconstrictor neurons innervating salivary glands, piloerector muscle, brown fat, or adrenergic chromaffin cells. Transneuronal tracing using pseudorabies virus demonstrated that many, but not all, preganglionic neurons in the vasoconstrictor pathway to forelimb skeletal muscle were CART immunoreactive. Similarly, analysis with the confocal microscope confirmed that 70% of boutons in contact with vasoconstrictor ganglion cells contained CART, whereas 30% did not. Finally, we show that CART-immunoreactive cells represented 69% of the preganglionic neuron population expressing c-Fos after systemic hypoxia. We conclude that CART is present in most, although not all, cardiovascular preganglionic neurons but not thoracic preganglionic neurons with non-cardiovascular targets. We suggest that CART immunoreactivity may identify the postulated "accessory" preganglionic neurons, whose actions may amplify vasomotor ganglionic transmission.