Interleukin-33 stimulates formation of functional osteoclasts from human CD14(+) monocytes.
Interleukin-33 stimulates formation of functional osteoclasts from human CD14(+) monocytes.
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DOI：
10.1007/s00018-010-0410-y
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发表时间：
2010-11
影响因子：
8
通讯作者：
Choi, Wahn Soo
中科院分区：
文献类型：
--
作者：
Mun, Se Hwan;Ko, Na Young;Kim, Hyuk Soon;Kim, Jie Wan;Kim, Do Kyun;Kim, A-Ram;Lee, Seung Hyun;Kim, Yong-Gil;Lee, Chang Keun;Lee, Seoung Hoon;Kim, Bo Kyung;Beaven, Michael A.;Kim, Young Mi;Choi, Wahn Soo
白细胞介素（IL）-33是最近描述的促炎细胞因子。在这里，我们证明了IL-33作为来自人CD 14+单核细胞的功能性破骨细胞（OC）的调节剂。IL-33刺激从单核细胞形成抗酒石酸酸性磷酸酶（TRAP）+多核OC。这种作用被抗ST 2抗体抑制，表明IL-33通过其受体ST 2发挥作用，而不是通过NF-κB配体受体激活剂（RANKL）诱饵、骨保护素或抗RANKL抗体发挥作用。IL-33刺激单核细胞中对OC发育至关重要的信号分子的活化磷酸化。这些包括Syk、磷脂酶Cγ2、Gab 2、MAP激酶、TAK-1和NF-κB。IL-33还增强OC分化因子的表达，包括TNF-α受体相关因子6（TRAF 6）、活化T细胞胞质核因子1、c-Fos、c-Src、组织蛋白酶K和降钙素受体。IL-33最终诱导骨吸收。这项研究表明，IL-33的破骨细胞生成特性是通过TRAF 6以及人CD 14+单核细胞中基于免疫受体酪氨酸的活化基序依赖性Syk/PLCγ途径介导的。
Interleukin (IL)-33 is a recently described pro-inflammatory cytokine. Here we demonstrate IL-33 as a regulator of functional osteoclasts (OCs) from human CD14+ monocytes. IL-33 stimulates formation of tartrate-resistant acid phosphatase (TRAP)+ multinuclear OCs from monocytes. This action was suppressed by anti-ST2 antibody, suggesting that IL-33 acts through its receptor ST2, but not by the receptor activator of NF-κB ligand (RANKL) decoy, osteoprotegerin, or anti-RANKL antibody. IL-33 stimulated activating phosphorylations of signaling molecules in monocytes that are critical for OC development. These included Syk, phospholipase Cγ2, Gab2, MAP kinases, TAK-1, and NF-κB. IL-33 also enhanced expression of OC differentiation factors including TNF-α receptor-associated factor 6 (TRAF6), nuclear factor of activated T cells cytoplasmic 1, c-Fos, c-Src, cathepsin K, and calcitonin receptor. IL-33 eventually induced bone resorption. This study suggests that the osteoclastogenic property of IL-33 is mediated through TRAF6 as well as the immunoreceptor tyrosine-based activation motif-dependent Syk/PLCγ pathway in human CD14+ monocytes.
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影响因子：
5
作者：
Iikura, Motoyasu;Suto, Hajime;Nakae, Susumu
通讯作者：
Nakae, Susumu
DOI：
10.1073/pnas.0401602101
发表时间：
2004-04-20
影响因子：
11.1
作者：
Mócsai, A;Humphrey, MB;Nakamura, MC
通讯作者：
Nakamura, MC
影响因子：
10.5
作者：
Shim, JH;Xiao, CC;Ghosh, S
通讯作者：
Ghosh, S
影响因子：
--
作者：
Palmer, Gaby;Talabot-Ayer, Dominique;Gabay, Cem
通讯作者：
Gabay, Cem
影响因子：
64.5
作者：
Lagasse, E;Weissman, IL
通讯作者：
Weissman, IL