Resveratrol and curcumin reduce the respiratory burst of Chlamydia-primed THP-1 cells
Resveratrol and curcumin reduce the respiratory burst of Chlamydia-primed THP-1 cells
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DOI：
10.1016/j.bbrc.2005.05.073
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发表时间：
2005-07-22
影响因子：
3.1
通讯作者：
Deby, C
中科院分区：
文献类型：
--
作者：
Deby-Dupont, G;Mouithys-Mickalad, A;Deby, C
细胞内细菌肺炎衣原体参与动脉粥样硬化的炎症过程。我们之前已经证明，肺炎梭菌感染的单核细胞（THP-1细胞）对与NADPH氧化酶（NOX）活性增加相关的呼吸爆发增加的刺激有反应。我们现在测试了作用于NOX亚基组装或蛋白激酶C （NOX活性的触发器）的药物。测定了罗布麻素、白藜芦醇、芦丁、槲皮素、姜黄素和生育酚。细胞与衣原体和该剂共预孵育19 h，然后用肉豆酸酯佛博尔刺激。通过测定过氧化氢的产量来监测NOX的活性。白藜芦醇和姜黄素(10（-4)-10(-6)M）是比罗布麻素更好的抑制剂。a-生育酚无活性，γ -生育酚抑制剂仅在10(-4)M时无活性。槲皮素是无活性的，芦丁是一个中等但显著的抑制剂。10(-6) M芦丁和槲皮素对白藜芦醇的抑制作用增强。因此，白藜芦醇和姜黄素似乎对动脉粥样硬化的治疗很有意义。(c) 2005爱思唯尔公司版权所有。
The intracellular bacterium Chlamydia pneumoniae is involved in the inflammation process of atherosclerosis. We previously demonstrated that C. pneumonia infected monocytes (THP-1 cells) responded to stimulation by an increased respiratory burst linked to an increased NADPH oxidase (NOX) activity. We now tested agents acting on the assembly of the NOX subunits or on protein kinase C, a trigger of NOX activity. Apocynin, resveratrol, rutin, quercetin, curcumin, and tocopherols were tested. The cells were pre-incubated with Chlamydia and the agent for 19 h, and then stimulated with phorbol myristate acetate. The NOX activity was monitored by measuring the hydrogen peroxide production. Resveratrol and curcumin (10(-4)-10(-6)M) were better inhibitors than apocynin. a-Tocopherol was inactive, and gamma-tocopherol inhibitor at 10(-4) M only. Quercetin was inactive, and rutin a moderate but significant inhibitor. The inhibition by resveratrol was increased by 10(-6) M rutin or quercetin. Resveratrol and curcumin thus appeared to be interesting for atherosclerosis treatment. (c) 2005 Elsevier Inc. All rights reserved.