Micro open-sandwich ELISA to rapidly evaluate thyroid hormone concentration from serum samples.
Micro open-sandwich ELISA to rapidly evaluate thyroid hormone concentration from serum samples.
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DOI：
10.4155/bio.10.125
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发表时间：
2010-10
期刊：
影响因子：
1.8
通讯作者：
K. N. Islam;M. Ihara;Jinhua Dong;Noriyuki Kasagi;Toshihiro Mori;H. Ueda
中科院分区：
文献类型：
--
作者：
K. N. Islam;M. Ihara;Jinhua Dong;Noriyuki Kasagi;Toshihiro Mori;H. Ueda
背景甲状腺素（T4）是诊断甲状腺功能最常用的甲状腺激素。为了阐明 T4 的快速、灵敏检测，我们使用从噬菌体展示库获得的抗 T4 抗体片段制作了基于微流体的非竞争性免疫检测芯片系统。结果基于检测两个抗体可变区V(H)和V(L)之间相互作用的抗原依赖性的开放夹心ELISA原理，我们可以检测到低于1ng/ml的T4。该测定还成功应用于评估健康个体血清中的总 T4 浓度。结论 这将是第一个使用直接从免疫小鼠中挑选的抗体片段构建的微开放夹心 ELISA。该系统将应用于多种诊断标志物的灵敏检测。
BACKGROUND Thyroxine (T4) is the most commonly measured thyroid hormone for the diagnosis of thyroid function. To elucidate a rapid and sensitive assay for T4, we made a microfluidics-based noncompetitive immunodetection chip system using anti-T4 antibody fragments obtained from a phage display library. RESULTS Based on the open-sandwich ELISA principle that detects antigen-dependency of the interaction between the two antibody variable regions V(H) and V(L), we could detect less than 1 ng/ml of T4. The assay was also successfully applied to evaluate total T4 concentration in the serum of healthy individuals. CONCLUSION This would be the first micro open-sandwich ELISA constructed with antibody fragments directly selected from immunized mice. The system will be applied to the sensitive detection of many diagnostic markers.