Growth factor combination for chondrogenic induction from human mesenchymal stem cell
Growth factor combination for chondrogenic induction from human mesenchymal stem cell
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DOI：
10.1016/j.bbrc.2004.06.029
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发表时间：
2004-07-30
影响因子：
3.1
通讯作者：
Bunyaratvej, A
中科院分区：
文献类型：
--
作者：
Indrawattana, N;Chen, GP;Bunyaratvej, A
在过去十年中，许多软骨工程策略不断涌现。干细胞诱导是软骨工程可能的方法之一。间充质干细胞（MSCs）因其多能性和易获取性已被证明是一种有吸引力的细胞来源。它需要细胞生长因子的刺激以使多能性MSCs分化为软骨细胞谱系。我们通过循环使用生长因子展示了在人骨髓间充质干细胞（hBMSCs）上体外诱导软骨形成的特定模式。制备hBMSCs的细胞团培养物用于软骨诱导。生长因子：转化生长因子 -β3、骨形成蛋白 - 6和胰岛素样生长因子 - 1联合用于细胞诱导。在细胞诱导后第21天对基因表达、组织学、免疫组织学和实时定量PCR方法进行检测。组织学和免疫组织学结果显示，在联合诱导和循环诱导这两种模式下，诱导细胞在形态上显示出软骨细胞的特征，在细胞外基质方面也符合软骨细胞特征。此外，实时定量PCR检测显示出软骨形成的基因标志物、Ⅱ型胶原蛋白和聚集蛋白聚糖的表达。这项研究表明，可以从骨髓间充质干细胞生成软骨组织。有趣的是，实时定量PCR检测显示，联合使用生长因子转化生长因子 -β3和骨形成蛋白 - 6或者转化生长因子 -β3和胰岛素样生长因子 - 1对软骨形成诱导更为有效。这些生长因子的组合可能是体外诱导软骨形成的重要关键。（C）2004爱思唯尔公司。保留所有权利。
During the last decade, many strategies for cartilage engineering have been emerging. Stem cell induction is one of the possible approaches for cartilage engineering. The mesenchymal stem cells (MSCs) with their pluripotency and availability have been demonstrated to be an attractive cell source. It needs the stimulation with cell growth factors to make the multipluripotent MSCs differentiate into chondrogenic lineage. We have shown particular patterns of in vitro chondrogenesis induction on human bone marrow MSCs (hBMSCs) by cycling the growth factors. The pellet cultures of hBMSCs were prepared for chondrogenic induction. Growth factors: TGF-beta3, BMP-6, and IGF-1 were used in combination for cell induction. Gene expression, histology, immunohistology, and real-time PCR methods were measured on days 21 after cell induction. As shown by histology and immunohistology, the induced cells have shown the feature of chondrocytes in their morphology and extracellular matrix in both inducing patterns of combination and cycling induction. Moreover, the real-time PCR assay has shown the expression of gene markers of chondrogenesis, collagen type II and aggrecan. This study has demonstrated that cartilage tissue can be created from bone marrow mesenchymal stem cells. Interestingly, the combined growth factors TGF-beta3 and BMP-6 or TGF-beta3 and IGF-1 were more effective for chondrogenesis induction as shown by the real-time PCR assay. The combination of these growth factors may be the important key for in vitro chondrogenesis induction. (C) 2004 Elsevier Inc. All rights reserved.