Nestin-Cre transgenic mouse line Nes-Cre1 mediates highly efficient Cre/IoxP mediated recombination in the nervous system, kidney, and somite-derived tissues
Nestin-Cre transgenic mouse line Nes-Cre1 mediates highly efficient Cre/IoxP mediated recombination in the nervous system, kidney, and somite-derived tissues
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DOI：
10.1002/dvg.20226
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发表时间：
2006-08-01
期刊：
影响因子：
1.5
通讯作者：
Trumpp, Andreas
中科院分区：
文献类型：
--
作者：
Dubois, Nicole C.;Hofmann, Denise;Trumpp, Andreas
在这里，我们描述了一代的Nes-Cre 1转基因小鼠系，其中Cre重组酶的表达是由大鼠巢蛋白启动子和内含子2增强子控制。该细胞系以前曾用于中枢神经系统和第一鳃弓外胚层中各种基因的条件性功能丧失研究。在这里，我们报告了详细的时间和空间重组模式的Nes-Cre 1使用三种不同的报告Cre介导的重组，ROSA 26 R（R26 R），Z/AP，Z/EG。Cre/loxP重组检测早在头折叠阶段的胚胎。到胚胎第15.5天，重组几乎发生在神经系统的所有细胞中，并且出乎意料地也发生在体节衍生的组织和肾脏中。很少或没有重组的组织包括心脏、肝脏、胸腺和肺。这项研究表明，Nes-Cre 1介导的重组发生在神经外胚层的祖细胞类型，发展中的中肾，体节。
Here we describe the generation of the Nes-Cre1 transgenic mouse line in which Cre recombinase expression is controlled by the rat nestin promoter and intron 2 enhancer. This line has previously been used for conditional loss-of-function studies of various genes in the central nervous system and first branchial arch ectoderm. Here we report the detailed temporal and spatial recombination pattern of Nes-Cre1 using three different reporters of Cre-mediated recombination, ROSA26R (R26R), Z/AP, and Z/EG. Cre/loxP recombination was detected in embryos as early as the head-fold stage. By embryonic day (E)15.5 recombination occurred in virtually all cells of the nervous system and unexpectedly also in somite-derived tissues and kidneys. Tissues with little or no recombination included heart, liver, thymus, and lung. This study suggests that Nes-Cre1-mediated recombination occurs in progenitor cell types present in the neuroectoderm, the developing mesonephros, and the somites.