Tissue Handling and Dissociation for Single-Cell RNA-Seq
Tissue Handling and Dissociation for Single-Cell RNA-Seq
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DOI：
10.1007/978-1-4939-9240-9_2
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发表时间：
2019-01-01
期刊：
影响因子：
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通讯作者：
Miragaia, Ricardo J.
中科院分区：
文献类型：
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作者：
Braga, Felipe A. Vieira;Miragaia, Ricardo J.
所有单细胞方案的起始材料都是细胞悬液。免疫细胞的特殊功能和空间分布通常使它们很容易从它们居住的组织中分离出来。在这里，我们描述了用于从淋巴组织和非淋巴组织中获得人类免疫细胞的组织分离方案，然后用作单细胞方法的输入。我们强调了可能影响单细胞数据最终质量的主要因素，即可能对其解释产生偏差的应力信号。
The starting material for all single-cell protocols is a cell suspension. The particular functions and spatial distribution of immune cells generally make them easy to isolate them from the tissues where they dwell. Here we describe tissue dissociation protocols that have been used to obtain human immune cells from lymphoid and nonlymphoid tissues to be then used as input to single-cell methods. We highlight the main factors that can influence the final quality of single-cell data, namely the stress signatures that can bias its interpretation.