The role of intracellular zinc in modulation of life and death of Hep-2 cells
The role of intracellular zinc in modulation of life and death of Hep-2 cells
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细胞内锌在调节 Hep-2 细胞生死中的作用
DOI：
10.1023/a:1020603110255
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发表时间：
2003
期刊：
影响因子：
3.5
通讯作者：
M. Červinka
中科院分区：
文献类型：
--
作者：
E. Rudolf;K. Rudolf;J. Radocha;J. Peychl;M. Červinka
用原子吸收分光光度法测定了不同培养条件下Hep-2细胞内锌浓度的变化。在含10%血清的DMEM中进行标准培养后，锌的平均浓度测定为0.88 ± 0.09 μg/mg蛋白质，暴露于低血清培养基的细胞中的值显著降低。接下来，研究了一系列生理和超生理浓度的硫酸锌对喉细胞的影响及其与确定的细胞内锌浓度的相关性。结果表明，当锌浓度超过100 μM时，Hep-2细胞在96 h内即出现明显的细胞毒性，细胞核选择性染色、caspase-3和特异性细胞角蛋白18片段的免疫荧光检测显示，锌浓度超过100 μM时，Hep-2细胞在96 h内即出现明显的细胞死亡。观察到两种类型的细胞死亡，分别在中等毒性锌浓度150 μM下诱导凋亡，在较高锌浓度300 μM和750 μM下诱导坏死。另一方面，较低浓度（1.5-100 μM）在相同时间间隔内未产生任何可测量的细胞形态和功能变化。1.5 μM的锌对Hep-2细胞的增殖有一定的促进作用，这可能与细胞内锌的最大耐受水平有关。这些结果说明，当试图表征外源性锌对喉细胞的生与死的影响时，确定细胞内锌水平的重要性。
Varying intracellular concentrations of zinc in laryngeal Hep-2 cells in relation to changing cultivation conditions in vitro were determined by atomic absorption spectrophotometry. Upon standard cultivation in DMEM with 10% serum, the mean concentration of zinc was determined at 0.88 ± 0.09 μg/mg protein, with substantially decreased values in the cells exposed to a low-serum medium. Next, the study of the effects of a series of physiological and supraphysiological concentrations of ZnSO4 on laryngeal cells and their correlation with determined intracellular concentrations of zinc was performed. It was found that zinc concentrations above 100 μM were toxic to Hep-2 cells, inducing cell death in the interval of 96 h as determined by videomicroscopy, selective nuclear staining, and immunofluorescence detection of caspase-3 and specific cytokeratin 18 fragment. Both types of cell death were observed, with apoptosis being induced at moderately toxic zinc concentration of 150 μM and necrosis at higher zinc concentrations of 300 μM and 750 μM, respectively. Lower concentrations (1.5–100 μM), on the other hand, did not produce any measurable changes in cell morphology and function in the same time interval. Zinc at concentration of 1.5 μM was found to slightly enhance proliferation of Hep-2 cells up to the certain time point, which seemed to correlate with maximal tolerable momentary intracellular level of zinc. These results illustrate the importance of determining the intracellular levels of zinc when trying to characterize the effect of exogenous zinc on life and death of laryngeal cells.
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严重缺锌对大鼠视网膜色素上皮形态的影响。
影响因子：
4.4
作者：
Leure-duPree,AE;McClain,CJ
通讯作者：
McClain,CJ
DOI：
10.1073/pnas.93.16.8395
发表时间：
1996-08
影响因子：
11.1
作者：
A. Takahashi;A. Takahashi;E. Alnemri;Y. Lazebnik;Y. Lazebnik;T. Fernandes‐Alnemri;G. Litwack;R. Moir;R. Goldman;G. Poirier;S. Kaufmann;W. Earnshaw;W. Earnshaw
通讯作者：
A. Takahashi;A. Takahashi;E. Alnemri;Y. Lazebnik;Y. Lazebnik;T. Fernandes‐Alnemri;G. Litwack;R. Moir;R. Goldman;G. Poirier;S. Kaufmann;W. Earnshaw;W. Earnshaw
DOI：
10.1093/ajcn/71.1.81
发表时间：
2000
期刊：
The American journal of clinical nutrition
影响因子：
--
作者：
P. Meerarani;P. Ramadass;M. Toborek;H. Bauer;H. Bauer;B. Hennig
通讯作者：
P. Meerarani;P. Ramadass;M. Toborek;H. Bauer;H. Bauer;B. Hennig
细胞凋亡中蛋白磷酸酶、线粒体细胞色素 c 释放和 caspase 激活之间的时间关系。
影响因子：
3.7
作者：
Wolf,CM;Eastman,A
通讯作者：
Eastman,A