HORMONAL-CONTROL OF 17BETA-ESTRADIOL BIOSYNTHESIS IN PROESTROUS RAT FOLLICLES - ESTRADIOL PRODUCTION BY ISOLATED THECA VERSUS GRANULOSA
HORMONAL-CONTROL OF 17BETA-ESTRADIOL BIOSYNTHESIS IN PROESTROUS RAT FOLLICLES - ESTRADIOL PRODUCTION BY ISOLATED THECA VERSUS GRANULOSA
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DOI：
10.1210/endo-102-1-227
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发表时间：
1978-01-01
期刊：
影响因子：
4.8
通讯作者：
ARMSTRONG, DT
中科院分区：
文献类型：
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作者：
FORTUNE, JE;ARMSTRONG, DT
卵泡17 β-雌二醇的细胞来源和促性腺激素控制研究雌二醇分泌。在LH [促黄体激素]（0.1 μ g/ml）、FSH [促卵泡激素]（0.1 μ g/ml）和/或睾酮（5 × 10 - 6）存在或不存在的情况下，将从用孕马血清促性腺激素处理的未成熟大鼠的动情前期卵泡分离的卵泡膜和颗粒分别培养3天。10-7 M）。在培养3、6、12、24、48和72 h时收集并更换培养基，并通过放射免疫测定法测定雌二醇。颗粒细胞培养物分泌的雌二醇在外源性雄激素前体的情况下可以忽略不计。在培养液中添加睾酮可显著增加颗粒细胞雌二醇的产生。只有当睾酮和FSH都包含在培养基中时，在整个3天的培养期内才能持续分泌雌二醇。在没有外源性睾酮的情况下，卵泡膜制剂分泌的雌二醇量相对较低，而在外源性睾酮存在的情况下，分泌的雌二醇量略高。促性腺激素对卵泡膜制备物的雌二醇产生无明显影响。在3天的培养期内，颗粒细胞培养物产生的雌二醇比卵泡膜制剂多约9倍。虽然颗粒细胞培养物似乎是均匀的，但卵泡膜制剂含有污染颗粒细胞的斑块。当比较两种不同类型的卵泡膜制剂时，一种被颗粒细胞污染，另一种几乎没有污染细胞，与含有颗粒细胞的制剂相比，清洁制剂的雌二醇分泌大大减少，可以忽略不计。卵泡膜雌二醇的表观产生可能部分或完全是由于污染细胞的产生。这些结果，连同以前的报告，LH刺激卵泡膜，但不是颗粒，分泌睾酮，是一致的假设，卵泡膜供应雄激素前体的颗粒细胞芳香化它雌二醇。
The cellular source and gonadotropic control of follicular 17.beta.-estradiol secretion were investigated. Theca and granulosa isolated from proestrous follicles of immature rats treated with pregnant mare''s serum gonadotropin were cultured separately for 3 days in the presence or absence of LH [luteinizing hormone] (0.1 .mu.g/ml), FSH [follicle stimulating hormone] (0.1 .mu.g/ml), and/or testosterone (5 .times. 10-7 M). Medium was collected and replaced at 3, 6, 12, 24, 48 and 72 h of culture and measured for estradiol by radioimmunoassay. Granulosa cell cultures secreted negligible quantities of estradiol in the absence of exogenous androgen precursor. Addition of testosterone to the culture medium greatly increased the production of estradiol by granulosa cells. Estradiol secretion was sustained throughout the 3-day culture period only if both testosterone and FSH were included in the culture medium. Thecal preparations secreted relatively low amounts of estradiol in the absence of exogenous testosterone and somewhat higher quantities in its presence. Gonadotropins had no apparent effect on estradiol production by thecal preparations. Granulosa cell cultures produced about 9-fold more estradiol during the 3-day culture period than did thecal preparations. While granulosa cell cultures appeared to be homogeneous, thecal preparations contained patches of contaminating granulosa cells. When 2 different types of thecal preparation, one contaminated with granulosa cells and the other almost free of contaminating cells, were compared, estradiol secretion by the cleaner preparations was greatly reduced and negligible compared to secretion by preparations containing granulosa cells. Apparent production of estradiol by the theca might be partly or solely due to production by contaminating cells. These results, together with previous reports that LH stimulates theca, but not granulosa, to secrete testosterone, are consistent with the hypothesis that the theca supplies androgen precursor to the granulosa cells which aromatize it to estradiol.