Physiological Effects of Retrograded, α-Amylase-Resistant Cornstarch in Rats
Physiological Effects of Retrograded, α-Amylase-Resistant Cornstarch in Rats
复制标题
逆行、抗 α-淀粉酶玉米淀粉对大鼠的生理影响
DOI：
10.1093/jn/121.1.44
复制
发表时间：
1991
影响因子：
4.2
通讯作者：
I. Johnson
中科院分区：
文献类型：
--
作者：
J. Gee;R. Faulks;I. Johnson
以高直链玉米淀粉为原料，经糊化处理，在1℃下贮藏48h，制得变性直链淀粉，干燥后以粉状半合成饲料饲喂雄性Wistar大鼠14d。对照组大鼠接受类似的饮食，用蔗糖代替抗性淀粉。在整个喂养期内进行粪便收集。14d后处死动物。取小肠和盲肠进行形态学检查、小肠隐窝细胞生成率(CCPR)测定和肠腔碳水化合物含量分析。采集血样进行胆固醇、胰高血糖素和肠高血糖素分析。饲喂淀粉的大鼠的粪便体积和淀粉排泄量均高于对照组，但随饲喂时间的延长而显著下降。饲喂淀粉的大鼠盲肠大小和内容物也较大，盲肠pH值显著降低。饲喂淀粉的大鼠回肠的CCPR高于对照组的66%(P<0.001)，而空肠的CCPR则无显著差异。血清胆固醇和肠高血糖素水平没有差异。我们的结论是，降解的直链淀粉在大鼠的消化道中被部分降解，但它对粪便体积的贡献很大。
Retrograded amylose was prepared by gelatinization of high amylose cornstarch, followed by storage at 1 degrees C for 48 h. The insoluble residue, which resisted hydrolysis with porcine amylase, was dried and fed to male Wistar rats for 14 d in powdered semisynthetic diet. Control rats received a similar diet containing sucrose in place of resistant starch. Fecal collections were performed throughout the feeding period. After 14 d the animals were killed. The small intestine and cecum were removed for morphological examination, measurement of small intestinal crypt cell production rate (CCPR) and analysis of luminal carbohydrate content. Blood samples were collected for analysis of cholesterol, glucagon, and enteroglucagon. In the starch-fed rats, fecal bulk and excretion of starch were higher than in the controls, but they declined markedly over the feeding period. Cecal size and contents were also greater in the starch-fed rats, and cecal pH was significantly lower. The CCPR was 66% higher in the ileum of the starch-fed rats (P less than 0.001), but there was no difference in the jejunum. There were no differences in serum cholesterol or enteroglucagon levels. We conclude that retrograded amylose is partially degraded in the alimentary tract of rats, but it contributes significantly to fecal bulk.