SPECTROPHOTOMETRIC ASSAY FOR SULFHYDRYL GROUPS USING N-ETHYLMALEIMIDE
SPECTROPHOTOMETRIC ASSAY FOR SULFHYDRYL GROUPS USING N-ETHYLMALEIMIDE
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DOI：
10.1021/ac60139a031
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发表时间：
1958-01-01
影响因子：
7.4
通讯作者：
ALEXANDER, NM
中科院分区：
文献类型：
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作者：
ALEXANDER, NM
7X t -乙基马来酰亚胺（NEM）在中性pH下与巯基化合物发生1 V快速反应（4,5）。用分光光度法测定了等摩尔量的ar -乙基马来酰亚胺与还原性谷胱甘肽的反应速率，前者在300mg时吸收率降低(5)，但在此条件下反应不完全。Roberts和Rouser(7)表明，当A'-乙酰亚胺过量存在时，300 mg时吸光度的变化与半胱氨酸和谷胱甘肽的浓度成正比。他们用这种方法测定了牛血清白蛋白与白蛋白的反应程度。V-ethyl-maleimide。
7X T-ethylmaleimide (NEM) reacts 1 V rapidly with sulhvdryl com-pounds at neutral pH (4, 5). The rate of reaction of equimolar amounts of Ar-ethylmaleimide and reduced glutathione has been followed spectrophotometrically by a decrease in absorption of the former at 300 mg (5), but the reac-tion does not go to completion under these conditions. Roberts and Rouser (7) showed that the change in absorb-ance at 300 mg is proportional to the concentrations of cysteine and glutathione when A'-ethvlmaleimide is present in excess. They used this method to determine the extent to which bovine serum albumin reacts with. V-ethyl-maleimide.