Involvement of histone acetylation in tomato resistance to Ralstonia solanacearum
Involvement of histone acetylation in tomato resistance to Ralstonia solanacearum
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组蛋白乙酰化参与番茄对青枯菌的抗性
DOI：
10.1016/j.scienta.2021.110163
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发表时间：
2021-04-05
影响因子：
4.3
通讯作者：
Li, Tao
中科院分区：
文献类型：
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作者：
Gong, Chao;Su, Huihui;Li, Tao
细菌性枯萎病（BW）是番茄（Solanum lycopersicum L.）青枯病菌Ralstonia solanacearum（R.青枯菌属）。虽然已经提出了几种策略来控制BW，但其分子机制在很大程度上是未知的。本研究对两个番茄品种JG-No3和XN 021进行了抗病和感病鉴定。青枯菌属（solanacearum）。抗病品种XN 021中多数防御相关基因表达上调，感病品种JG-No3中多数防御相关基因表达下调。与JG-No3相比，XN 021中一些组蛋白脱乙酰酶（HDAC）的表达降低。此外，使用HDACs抑制剂TSA（曲古抑菌素A）处理的JG-No3中的疾病症状减轻。使用VIGS沉默SlHDA 6、SlHDT 1、SlHDT 2、SlSRT 1和SlSRT 2引起感病品种JGNo 3中的发病率降低。此外，两个品种的防御相关基因组蛋白H3和H4乙酰化水平在R.青枯菌感染，尤其是XN 021。总的来说，我们的研究结果表明，组蛋白乙酰化介导的HDAC在番茄BW抗性中起着重要作用。这项研究为BW疾病反应机制提供了新的见解。
Bacterial wilt (BW) is the most devastating vascular disease in tomato (Solanum lycopersicum L.), which caused by Ralstonia solanacearum (R. solanacearum). Although several strategies have been proposed to control BW, the molecular mechanisms are largely unknown. Here, we identify two tomato cultivars JG-No3 and XN021, which showed susceptible and resistant to R. solanacearum, respectively. Most defense-related genes were up-regulated in resistant cultivar XN021, whereas down-regulated in susceptible cultivar JG-No3. The expression of some histone deacetylases (HDACs) was reduced in XN021 compared to JG-No3. Furthermore, the disease symptoms were decreased in JG-No3 using the HDACs inhibitor, TSA (Trichostatin A) treatment. Silencing of SlHDA6, SlHDT1, SlHDT2, SlSRT1 and SlSRT2 using VIGS caused reducing disease incidence in susceptible cultivar JGNo3. In addition, histone H3 and H4 acetylation levels of defense-related genes were increased in both cultivars under R. solanacearum infection, especially XN021. Collectively, our results demonstrate that histone acetylation mediating by HDACs plays important roles in BW resistance in tomato. This study provides a new insight into the mechanism of disease response in BW.