Down-regulation of miR-146b-5p by long noncoding RNA MALAT1 in hepatocellular carcinoma promotes cancer growth and metastasis.
Down-regulation of miR-146b-5p by long noncoding RNA MALAT1 in hepatocellular carcinoma promotes cancer growth and metastasis.
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长链非编码RNA MALAT1下调miR-146b-5p在肝细胞癌中促进癌症生长和转移
DOI：
10.18632/oncotarget.15640
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发表时间：
2017-04-25
期刊：
影响因子：
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通讯作者：
Liu C
中科院分区：
文献类型：
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作者：
Li C;Miao R;Liu S;Wan Y;Zhang S;Deng Y;Bi J;Qu K;Zhang J;Liu C
microrna在肝癌的发生和发展中起着重要作用。在这项研究中，我们发现miR-146b-5p的下调与肝细胞癌（HCC）患者的肿瘤生长、转移和生存不良相关。miR-146b-5p在体外和体内均能抑制细胞增殖、迁移和侵袭，诱导细胞凋亡。值得注意的是，TNF受体相关因子6 （TRAF6）被证实是miR-146b-5p在HCC中的直接靶点，miR-146b-5p通过抑制TRAF6介导的Akt磷酸化发挥抑瘤作用。此外，我们发现长链非编码RNA MALAT1作为miR-146b-5p的分子海绵下调其在HCC中的表达。总的来说，我们的研究结果表明，miR-146b-5p通过靶向TRAF6介导的Akt磷酸化来抑制HCC的肿瘤生长和转移。
MicroRNAs play an important role in liver cancer genesis and progression. In this study, we identified down-regulation of miR-146b-5p associated with tumor growth, metastasis and poor survival in hepatocellular carcinoma (HCC) patients. miR-146b-5p could suppress proliferation, migration, and invasion and induced apoptosis in vitro and in vivo. Remarkably, TNF receptor associated factor 6 (TRAF6) was confirmed as a direct target of miR-146b-5p in HCC and miR-146b-5p exerted the tumor suppression roles through inhibiting the phosphorylation of Akt mediated by TRAF6. Furthermore, we identified long non-coding RNA MALAT1 as a molecular sponge of miR-146b-5p to down-regulate its expression in HCC. In general, our results indicate that miR-146b-5p inhibits tumor growth and metastasis of HCC by targeting TRAF6 mediated Akt phosphorylation.