Preparation and Characterization of NH2-Terminal Fibrinogen Bβ Fragments from N-DSK of Human Fibrinogen
Preparation and Characterization of NH2-Terminal Fibrinogen Bβ Fragments from N-DSK of Human Fibrinogen
复制标题
人纤维蛋白原 N-DSK NH2 末端纤维蛋白原 Bβ 片段的制备和表征
DOI：
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发表时间：
1984
影响因子：
6.7
通讯作者：
R. Canfield
中科院分区：
文献类型：
--
作者：
S. Birken;G. Agosto;B. Lahiri;R. Canfield
摘要 为了研究纤维蛋白原 Bβ 链 NH2 末端质片段的早期释放，需要大量的 Bβ 1-42 和 Bβ 1-21 作为免疫原、放射免疫测定标准品以及输注到人类志愿者体内以确定这些肽的半衰期。为此，已经开发了采用从纤维蛋白原选择性蛋白水解切割这些片段的方法。由 CNBr 切割纤维蛋白原产生的 N-DSK 片段和 Bβ 1-118 都被用作纤溶酶的底物，发现 N-DSK 的产率更高。 Bβ 1-42 和 Bβ 1-21 通过凝胶过滤和 SP-Sephadex 离子交换色谱法使用挥发性缓冲液进行纯化。当 Bβ 1-42 的纯化制剂在反相高效液相色谱上进行色谱分析时，相同氨基酸组成的两个峰被分离，可能是由于焦谷氨酸或酰胺的差异。
Summary In order to investigate the early release of NH2-terminal plasmic fragments from the Bβ chain of fibrinogen, substantial quantities of Bβ 1-42 and Bβ 1-21 are required as immunogens, as radioimmunoassay standards and for infusion into human volunteers to determine the half-lives of these peptides. Towards this end methods that employ selective proteolytic cleavage of these fragments from fibrinogen have been developed. Both the N-DSK fragment, produced by CNBr cleavage of fibrinogen, and Bβ 1-118 were employed as substrates for plasmin with the finding of higher yields from N-DSK. Bβ 1-42 and Bβ 1-21 were purified by gel filtration and ion-exchange chromatography on SP-Sephadex using volatile buffers. When the purified preparation of Bβ 1-42 was chromatographed on reverse-phase high performance liquid chromatography, two peaks of identical amino acid composition were separated, presumably due either to pyroglutamate or to amide differences.
人纤维蛋白原 b β 链 NH2 末端的纤溶酶蛋白水解序列。
影响因子：
2.9
作者：
Koehn,JA;Hurlet-Jensen,A;Nossel,HL;Canfield,RE
通讯作者：
Canfield,RE