A novel membrane-bound E3 ubiquitin ligase enhances the thermal resistance in plants
A novel membrane-bound E3 ubiquitin ligase enhances the thermal resistance in plants
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一种新型膜结合 E3 泛素连接酶增强植物的耐热性
DOI：
10.1111/pbi.12120
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发表时间：
2014-01-01
影响因子：
13.8
通讯作者：
Yang, Yi
中科院分区：
文献类型：
--
作者：
Liu, Zhi-Bin;Wang, Jian-Mei;Yang, Yi
高温胁迫破坏细胞内稳态，导致作物生长发育严重迟缓。因此，揭示植物应对热胁迫的机制具有重要意义。在这项研究中，我们发现了一个新的基因，我们从甘蓝型油菜，简称为BnTR1，在植物热胁迫反应中发挥关键作用。BnTR 1是一种膜结合RINGv（C4 HC 3）蛋白，在体外表现出E3连接酶活性。我们证明了BnTR 1的适度表达足以使不利的环境影响最小化，并赋予发育耐热性，而不会对B.napus和Oryza sativa产生任何有害影响。对BnTR1作用机制的研究表明，BnTR1可能通过调节钙通道的活性，参与介导Ca2+的动态变化，进而改变植物热激因子和热激蛋白的转录水平，从而提高植物的耐热性。因此，我们的研究确定BnTR1作为一个新的关键因素的保守机制赋予植物的耐热性。
High temperature stress disturbs cellular homoeostasis and results in a severe retardation in crop growth and development. Thus, it is important to reveal the mechanism of plants coping with heat stress. In this study, a novel gene that we identified from Brassica napus, referred to as BnTR1, was found to play a key role in heat stress response in planta. BnTR1 is a membrane-bound RINGv (C4HC3) protein that displays E3 ligase activity in vitro. We demonstrated that modest expression of BnTR1 is sufficient to minimize adverse environmental influence and confers thermal resistance on development without any detrimental effects in B.napus and Oryza sativa. Our investigation into the action mechanism indicates that BnTR1 is likely to be involved in mediating Ca2+ dynamics by regulating the activity of calcium channels, which further alters the transcripts of heat shock factors and heat shock proteins contributing to plant thermotolerance. Hence, our study identified BnTR1 as a novel key factor underlying a conserved mechanism conferring thermal resistance in plants.