Transmission of tuberculosis within family-households
Transmission of tuberculosis within family-households
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DOI：
10.1016/j.jinf.2011.12.022
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发表时间：
2012-06-01
影响因子：
28.2
通讯作者：
Zwolska, Zofia
中科院分区：
文献类型：
--
作者：
Augustynowicz-Kopec, Ewa;Jagielski, Tomasz;Zwolska, Zofia
目的：20 世纪 90 年代引入分子分型方法来研究结核病 (TB) 流行病学，显着提高了量化结核分枝杆菌在不同人类环境中传播的可能性。本研究的目的是调查波兰 35 个家庭中结核病的传播。方法：使用两种基于 PCR 的基因分型方法：spoligotyping 和分枝杆菌散布重复单元可变串联重复次数 (MIRU-VNTR) 分型。结果：在 78 名患者中，49 名 (63%) 可以根据组合分型后的相同 DNA 指纹被分配为家庭内传播方法。然而，如果在簇定义中可以容忍单个 spoligotype 间隔区或单个 MIRU-VNTR 基因座变异，则所有患者的家庭内传播率将上升至 85%。对于 6 个家庭的 12 名患者，结核分枝杆菌分离株在 spoligotyping 或 VNTR 分型或两种基因分型方法中均明显不同，表明这些患者是由社区来源感染的。 结论：这项研究首次提供了在波兰家庭中进行的分子流行病学调查的结果。它表明家庭环境是结核分枝杆菌传播的重要储存库，因此支持对结核病患者的家庭接触者进行常规和广泛的筛查。 (c) 2012 年英国感染协会。由爱思唯尔有限公司出版。保留所有权利。
Objective: The introduction of molecular typing methods in the 1990s to study the epidemiology of tuberculosis (TB) has significantly improved the possibilities of quantifying transmission of Mycobacterium tuberculosis in different human settings. The purpose of this study was to investigate transmission of TB in 35 family-households in Poland.Methods: Two PCR-based genotyping methods: spoligotyping and mycobacterial interspersed repetitive unit-variable number of tandem repeats (MIRU-VNTR) typing were used.Results: Of 78 patients, 49 (63%), could be assigned to intra-household transmission on the basis of identical DNA fingerprints upon a combined typing approach. However, if a single spoligotype spacer or a single MIRU-VNTR locus variation was tolerated in the cluster definition, the intra-household transmission raised to 85% of all patients. For 12 patients in 6 households, the M. tuberculosis isolates were clearly distinct in either spoligotyping or VNTR typing or in both genotyping methods, suggesting that these patients were infected by the sources in the community.Conclusions: This study is the first to provide the results of a molecular epidemiological investigation performed within family-households in Poland. It shows the household setting as an important reservoir of M. tuberculosis transmission, and thus argues in favor of routine and extensive screening of the family contacts of TB patients. (c) 2012 The British Infection Association. Published by Elsevier Ltd. All rights reserved.