Estimating the time of skeletal muscle contusion based on the spatial distribution of neutrophils: a practical approach to forensic problems
Estimating the time of skeletal muscle contusion based on the spatial distribution of neutrophils: a practical approach to forensic problems
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DOI：
10.1007/s00414-021-02690-0
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发表时间：
2021-09-13
影响因子：
2.1
通讯作者：
Sun，Jun-hong
中科院分区：
文献类型：
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作者：
Du，Qiu-xiang;Wang，Liang;Sun，Jun-hong
这项研究旨在探索用于估计挫伤骨骼肌组织学年龄的中性粒细胞的空间分布，并评估各种指标的准确性，如中性粒细胞的比例、中性粒细胞平均距离和中性粒细胞在“连续轮廓线”区域的分布。5 5只SD大鼠随机分为对照组和挫伤组，伤后1，1.5，2，3，4，6h，伤后1，3，5，15d(n= 5)。苏木精-伊红(HE)染色和免疫组织化学(IHC)染色检测细胞核和中性粒细胞。于伤后0~24小时，测定距邻近血管100、200、300、400、500、600微米处中性粒细胞的分布，筛选最佳样本估计创面年龄。为了尽可能准确地估计创面年龄，对中性粒细胞比例、中性粒细胞平均距离和中性粒细胞分布进行了Fisher判别分析，原始病例和交叉验证病例的正确分类正确率分别为100.0%和95.0%。中性粒细胞在距邻近血管不同距离的空间分布与挫伤骨骼肌的组织学年龄密切相关，结合中性粒细胞比例、中性粒细胞平均距离和中性粒细胞分布可以准确估计损伤年龄。
The study aimed to explore the neutrophil’s spatial distributions used to estimate the histological age of contused skeletal muscle, and assessed the accuracy of various indicators, such as the proportion of neutrophils, “neutrophil mean distance,” and distribution of neutrophils in areas of “contiguous contour lines.” Fifty-five Sprague–Dawley rats were divided randomly into a control group and contusion groups at 1, 1.5, 2, 3, 4, and 6 h, as well as 1, 3, 5, and 15 days, post-injury (n= 5 per group). Nuclei and neutrophils were detected by hematoxylin and eosin (HE) staining and immunohistochemical (IHC) staining. At 0–24 h after injury, the distribution of neutrophils at distances of 100, 200, 300, 400, 500, and 600 µm from adjacent blood vessels was determined, and the best samples were screened to estimate wound age. To estimate wound age as accurately as possible, Fisher discriminant analysis (FDA) of the proportion of neutrophils, neutrophil mean distance, and distribution of neutrophils was performed, and 100.0% and 95.0% of the original and cross-validated cases were correctly classified, respectively. The spatial distribution of neutrophils at different distances from adjacent blood vessels showed a strong correlation with the histological age of contusion skeletal muscle, and the combination of the proportion of neutrophils, neutrophil mean distance, and distribution of neutrophils could be used to accurately estimate wound age.