Adventitial Cells Do Not Contribute to Neointimal Mass After Balloon Angioplasty of the Rat Common Carotid Artery
Adventitial Cells Do Not Contribute to Neointimal Mass After Balloon Angioplasty of the Rat Common Carotid Artery
复制标题
DOI：
10.1161/circ.104.14.1591
复制
发表时间：
2001-10
期刊：
影响因子：
--
通讯作者：
H. De León;J. Ollerenshaw;K. Griendling;J. Wilcox
中科院分区：
文献类型：
--
作者：
H. De León;J. Ollerenshaw;K. Griendling;J. Wilcox
背景-以前的研究表明，球囊血管成形术后外膜细胞迁移到新生内膜可能在血管病变形成中起重要作用。本实验旨在研究大鼠颈动脉机械损伤后外膜细胞的迁移。方法和结果：在大鼠颈总动脉球囊血管成形术后，用PKH 26（一种荧光染料）对外膜细胞进行原位染色。在不同时间点处死动物，并在光学和荧光显微镜下检查组织切片。PKH 26标记的细胞仅在外膜中检测到。在检查的任何时间点，中膜或新生内膜中均不存在标记细胞。由肌成纤维细胞组成的高度细胞化的新外膜层在损伤后3天在整个外膜圆周上表现出广泛的增殖反应。结论：尽管外膜肌成纤维细胞在血管成形术后的重塑过程中起着重要作用，但在大鼠颈动脉血管成形术模型中，它们并不迁移到中膜或内膜层。
Background—Previous studies suggest that the migration of adventitial cells into the neointima after balloon angioplasty might have an important role in vascular lesion formation. The current experiments were designed to study the migration of adventitial cells in response to mechanical injury of the rat carotid artery. Methods and Results—Adventitial cells were stained in situ with PKH26, a fluorescent dye, after balloon angioplasty of the rat common carotid artery. Animals were killed at different time points, and tissue sections were examined under light and fluorescence microscopy. PKH26-labeled cells were detected exclusively in the adventitia. No labeled cells were present in the media or the neointima at any time point examined. A highly cellular neoadventitial layer composed of myofibroblasts exhibited an extensive proliferative response 3 days after injury over the entire adventitial circumference. Conclusions—Despite the prominent role that adventitial myofibroblasts seem to have in the postangioplasty remodeling process, they do not migrate to the medial or intimal layers in the rat carotid artery angioplasty model.