Molecular design of the α-keratin composite:: insights from a matrix-free model, hagfish slime threads
Molecular design of the α-keratin composite:: insights from a matrix-free model, hagfish slime threads
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DOI：
10.1098/rspb.2003.2591
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发表时间：
2004-02-07
影响因子：
4.7
通讯作者：
Gosline, JM
中科院分区：
文献类型：
--
作者：
Fudge, DS;Gosline, JM
我们进行了机械测试无基质角蛋白模型-盲鳗粘液线程-测试的假设，即中间丝（IF）在水合硬α-角蛋白保持在部分脱水状态。该假设预测干燥IF应具有与水合硬α-角蛋白的性质类似的机械性质，并且应比硬α-角蛋白在水中溶胀更多。机械和膨胀测量的盲鳗线程与这两个预测是一致的，这表明弹性体角蛋白基质抵抗IF膨胀，并保持IF刚度和屈服应力高。无基质IF（即粘液丝）无法从屈服后变形中恢复，这间接支持了基质的弹性性质。我们提出了一个一般的概念模型的IF为基础的材料，预测的刚度，屈服应力和延伸性的水合和交联的影响的结构力学。
We performed mechanical tests on a matrix-free keratin model-hagfish slime threads-to test the hypothesis that intermediate filaments (IFs) in hydrated hard alpha-keratins are maintained in a partly dehydrated state. This hypothesis predicts that dry IFs should possess mechanical properties similar to the properties of hydrated hard alpha-keratins, and should swell more than hard alpha-keratins in water. Mechanical and swelling measurements of hagfish threads were consistent with both of these predictions, suggesting that an elastomeric keratin matrix resists IF swelling and keeps IF stiffness and yield stress high. The elastomeric nature of the matrix is indirectly supported by the inability of matrix-free IFs (i.e. slime threads) to recover from post-yield deformation. We propose a general conceptual model of the structural mechanics of IF-based materials that predicts the effects of hydration and cross-linking on stiffness, yield stress and extensibility.