ACTH is a potent regulator of gene expression in human adrenal cells.
ACTH is a potent regulator of gene expression in human adrenal cells.
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DOI：
10.1677/jme-10-0006
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发表时间：
2010-07
影响因子：
3.5
通讯作者：
Rainey WE
中科院分区：
文献类型：
--
作者：
Xing Y;Parker CR;Edwards M;Rainey WE
肾上腺是盐皮质激素、糖皮质激素和所谓的肾上腺雄激素的主要来源。在生理条件下，皮质醇和肾上腺雄激素的合成主要由促肾上腺皮质激素（ACTH）控制。虽然已经确定ACTH可以刺激类固醇生成，但ACTH对人肾上腺细胞中整体基因表达的影响尚未确定。在这项研究中，我们确定了慢性ACTH治疗对成人肾上腺（AA）和胎儿肾上腺（FA）细胞原代培养物中基因表达的影响。微阵列分析表明，48小时的促肾上腺皮质激素治疗导致30个AA基因和84个AA基因增加超过4倍，与20个基因共同在两种细胞培养物。这些基因中有6个编码参与类固醇生物合成的酶，ACTH受体及其辅助蛋白，MRAP（ACTH受体辅助蛋白）。这些数据提供了一组ACTH调节基因，包括许多以前没有研究过的肾上腺功能。这些基因代表调节肾上腺分化和类固醇激素生物合成的候选基因。
The adrenal glands are the primary source of minerocorticoids, glucocorticoids and the so-called adrenal androgens. Under physiological conditions cortisol and adrenal androgen synthesis are controlled primarily by adrenocorticotrophic hormone (ACTH). Although it has been established that ACTH can stimulate steroidogenesis, the effects of ACTH on overall gene expression in human adrenal cells have not been established. In this study, we defined the effects of chronic ACTH treatment on global gene expression in primary cultures of both adult adrenal (AA) and fetal adrenal (FA) cells. Microarray analysis indicated that 48 h of ACTH treatment caused 30 AA genes and 84 AA genes to increase by greater than 4-fold, with 20 genes common in both cell cultures. Among these genes were six encoding enzymes involved in steroid biosynthesis, the ACTH receptor and its accessory protein, MRAP (ACTH receptor accessory protein). These data provide a group of ACTH-regulated genes including many that have not previously been studied with regard to adrenal function. These genes represent candidates for regulation of adrenal differentiation and steroid hormone biosynthesis.