INVOLVEMENT OF THE CYCLIC AMP-RESPONSIVE ELEMENT-BINDING PROTEIN IN BOVINE LEUKEMIA-VIRUS EXPRESSION IN-VIVO
INVOLVEMENT OF THE CYCLIC AMP-RESPONSIVE ELEMENT-BINDING PROTEIN IN BOVINE LEUKEMIA-VIRUS EXPRESSION IN-VIVO
复制标题
DOI：
10.1128/jvi.68.9.5845-5853.1994
复制
发表时间：
1994-09-01
影响因子：
5.4
通讯作者：
WILLEMS, L
中科院分区：
文献类型：
--
作者：
ADAM, E;KERKHOFS, P;WILLEMS, L
TAR 元件（Tax 响应元件；也称为 TxRE）是牛白血病病毒 (BLV) 表达调节的主要决定因素。为了深入了解病毒表达的机制，通过凝胶阻滞测定分析了蛋白质和 TAR 增强子 DNA 之间形成的复合物。我们在此报道，离体分离的 B 淋巴细胞的核裂解物含有与 TAR 特异性结合的蛋白质。针对环 AMP 响应元件结合 (CREB) 蛋白的抗体可超移仅存在于 BLV 感染的 B 淋巴细胞中的复合物 (C1)。因此，CREB 蛋白似乎是参与 BLV 体内表达的主要转录因子。
The TAR element (Tax-responsive element; also called TxRE) is a major determinant of the regulation of bovine leukemia virus (BLV) expression. In order to gain insight into the mechanisms of viral expression, complexes formed between proteins and the TAR enhancer DNA were analyzed by gel retardation assays. We report here that nuclear lysates from ex vivo-isolated B lymphocytes contain proteins that specifically bind to TAR. An antibody directed toward the cyclic AMP-responsive element binding (CREB) protein supershifted a complex (C1) present only in BLV-infected B lymphocytes. The CREB protein thus appears to be a major transcription factor involved in BLV expression in vivo.