Analysis of free radical production capacity in mouse faeces and its possible application in evaluating the intestinal environment: a pilot study
Analysis of free radical production capacity in mouse faeces and its possible application in evaluating the intestinal environment: a pilot study
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DOI：
10.1038/s41598-019-56004-x
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发表时间：
2019-12
影响因子：
4.6
通讯作者：
Yoshihisa Wakita;A. Saiki;H. Kaneda;S. Segawa;Y. Tsuchiya;Hiromi Kameya;S. Okamoto
中科院分区：
文献类型：
--
作者：
Yoshihisa Wakita;A. Saiki;H. Kaneda;S. Segawa;Y. Tsuchiya;Hiromi Kameya;S. Okamoto
肠道环境和宿主之间的复杂相互作用引起了相当大的关注，并且已经就肠道微生物组和代谢组进行了充分的研究。超氧化物和羟基自由基（·OH）等氧自由基是在正常细胞代谢过程中产生的。它们对真核细胞和原核细胞都有毒性，因此可能影响肠道内稳态。然而，氧自由基对肠道环境的影响尚未得到广泛研究。在此，我们应用电子自旋共振光谱和自旋捕获试剂来评估小鼠肠腔和粪便中的氧自由基产生能力。·OH在小肠和大肠的粪便和管腔中产生。粪便和大肠中的·OH水平无显著差异，提示粪便可作为替代样品用于估计结肠内容物中·OH的产生能力。然后，我们比较了五种不同小鼠品系（ddY，ICR，C57 BL/6，C3 H/HeJ和BALB/c）的粪便样品中的自由基水平，发现品系ddY的水平明显高于其他四种品系。此外，菌株ddY对葡聚糖硫酸钠诱导的结肠炎更敏感。这些差异可能与肠道细菌群Arthromitus的相对丰度有关，已知该细菌群可调节宿主免疫反应。从这些结果，我们认为，在小鼠粪便中的氧自由基的生产能力与肠道内稳态。
Complex interplay between the intestinal environment and the host has attracted considerable attention and has been well studied with respect to the gut microbiome and metabolome. Oxygen free radicals such as superoxide and the hydroxyl radical (•OH) are generated during normal cellular metabolism. They are toxic to both eukaryotic and prokaryotic cells and might thus affect intestinal homeostasis. However, the effect of oxygen free radicals on the intestinal environment has not been widely studied. Herein, we applied electron spin resonance spectroscopy with spin trapping reagents to evaluate oxygen free radical production capacity in the intestinal lumen and the faeces of mice.•OH was generated in faeces and lumens of the small and large intestines. There were no remarkable differences in•OH levels between faeces and the large intestine, suggesting that faeces can be used as alternative samples to estimate the•OH production capacity in the colonic contents. We then compared free radical levels in faecal samples among five different mouse strains (ddY, ICR, C57BL/6, C3H/HeJ, and BALB/c) and found that strain ddY had considerably higher levels than the other four strains. In addition, strain ddY was more susceptible to dextran sulphate sodium-induced colitis. These differences were possibly related to the relative abundance of the gut bacterial groupCandidatusArthromitus, which is known to modulate the host immune response. From these results, we suggest that the production capacity of oxygen free radicals in mouse faeces is associated with intestinal homeostasis.