Development of primer sets for a multiplex and a qPCR assays targeting Skeletonema species and its application to field samples
Development of primer sets for a multiplex and a qPCR assays targeting Skeletonema species and its application to field samples
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针对骨条菌物种的多重和 qPCR 检测引物组的开发及其在现场样品中的应用
DOI：
10.1007/s10872-018-00504-3
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发表时间：
2019
影响因子：
2.3
通讯作者：
K.
中科院分区：
文献类型：
--
作者：
1.Enjoji;N. Katano;T.;Yoshinaka;Y.;Furuoka;F.;Ando;Y.;Yamada;M.;Hamasaki;T.;Miyamura;E.;Otsubo;M.;Yokoyama;K.
硅藻是一种占优势的硅藻，特别是在沿海沃茨。然而，在自然环境中的物种识别和量化尚未建立，因为物种识别几乎是不可能的光学显微镜。在本研究中，我们开发了多重和实时PCR的引物组，以确定和计数的10个水母类，并应用该技术从春季到夏季的有明海的筑后江及其附近的沃茨的潮间带样品。调查期间共发现8种浮游植物。在这八个人中，S。3个站位均检测到marinoi-dohrniiccomplex，其次是河流刺线虫，主要分布在筑后川。在筑后河的潮带上游，盐度< 2，检测到的物种总数较低。这些结果揭示了微咸水环境中浮游植物的多样性，本研究设计的引物对分析浮游植物的种类组成是有用的。
Skeletonemais a dominant diatom, especially in coastal waters. However, species identification and quantification in the natural environment has not yet been established, as species identification can be nearly impossible with light microscopy. In the present study, we developed primer sets for multiplex and real-time PCR to identify and enumerate the 10Skeletonemaspecies, and applied this technique to samples obtained from the tidal zone of the Chikugo River and its adjacent waters of the Ariake Sea from spring to summer. In total, eightSkeletonemaspecies were detected during the investigation. Among the eight, theS. marinoi–dohrniicomplex was detected at all three stations.Skeletonema potamoswas the second most frequently detected, mainly in the Chikugo River. The overall number of detected species was low at the upper tidal zone of the Chikugo River, where salinity was < 2. These results reveal the diverseSkeletonemacommunity in the brackish environment, and the primer sets designed in the present study are useful for analyzing species composition ofSkeletonema.