Neisseria meningitidis GNA2132, a heparin-binding protein that induces protective immunity in humans
Neisseria meningitidis GNA2132, a heparin-binding protein that induces protective immunity in humans
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DOI：
10.1073/pnas.0915162107
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发表时间：
2010-02-23
影响因子：
11.1
通讯作者：
Arico, Beatrice
中科院分区：
文献类型：
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作者：
Serruto, Davide;Spadafina, Tiziana;Arico, Beatrice
GNA2132是一种功能未知的脑膜炎奈瑟菌抗原，通过反向疫苗学发现，已在动物模型中显示可诱导杀菌抗体。在这里，我们表明这种抗原在人体内诱导保护性免疫，并被脑膜炎球菌病后患者的血清识别。在体外，该蛋白通过富含精氨酸的区域与肝素结合，这种特性与人血清中未被包裹的细菌的存活率增加有关。此外，两种蛋白酶，脑膜炎球菌NalP和人乳铁蛋白，分别从富含精氨酸的区域上游和下游切割蛋白质。我们认为GNA2132是脑膜炎奈瑟菌重要的保护性抗原，建议将其命名为奈瑟菌肝素结合抗原（Neisserial Heparin Binding antigen， NHBA）。
GNA2132 is a Neisseria meningitidis antigen of unknown function, discovered by reverse vaccinology, which has been shown to induce bactericidal antibodies in animal models. Here we show that this antigen induces protective immunity in humans and it is recognized by sera of patients after meningococcal disease. The protein binds heparin in vitro through an Arg-rich region and this property correlates with increased survival of the unencapsulated bacterium in human serum. Furthermore, two proteases, the meningococcal NalP and human lactoferrin, cleave the protein upstream and downstream from the Arg-rich region, respectively. We conclude that GNA2132 is an important protective antigen of N. meningitidis and we propose to rename it, Neisserial Heparin Binding Antigen (NHBA).