Response of human pulps following acid conditioning and application of a bonding agent in deep cavities
Response of human pulps following acid conditioning and application of a bonding agent in deep cavities
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DOI：
10.1016/s0109-5641(01)00089-6
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发表时间：
2002-11-01
期刊：
影响因子：
5
通讯作者：
Teixeira, HM
中科院分区：
文献类型：
--
作者：
Costa, CAD;do Nascimento, ABL;Teixeira, HM
目标。本研究的目的是评估单瓶粘接剂系统应用于蚀刻或未蚀刻的深牙本质时，人牙髓的反应。18种V类深腔制剂分为3组：1组-全蚀刻+ 2层单键(SB) +复合树脂（Z-100）；第2组-搪瓷蚀刻+两层SB + Z-100，第3组-空腔地板内衬氢氧化钙衬里(Dycal) +搪瓷和侧壁酸蚀+两层SB + Z-100。2颗牙作为完整对照组。30天后拔牙，进行H、E、马松三色、布朗和布伦染色。1组牙本质残余厚度（RDT）小于300 mm，出现中度炎症反应，牙髓组织紊乱，反应性牙本质薄层沉积。这些组织学发现似乎与长树脂标签的形成和结合剂通过牙本质小管的扩散有关。在第二组中，只有一颗牙有轻微的炎症反应，RDT为162。第3组所有牙齿的组织学特征与完整对照组相似。细菌的存在与牙髓反应的强度无关。患者在实验过程中没有出现任何症状。影像学检查未见任何牙根尖周病变，意义及结论。酸蚀深牙本质（RDT小于300 μ m）衬有SB比未酸蚀深牙本质引起更强烈的牙髓反应。根据本研究观察到的结果和进行研究的条件，我们建议在腐蚀深层牙本质之前使用生物相容性衬垫，并使用SB (C) 2002年牙科材料学会。Elsevier Science Ltd.出版。版权所有。
Objectives. The aim of this in vivo study was to evaluate the human dental pulp response when a one-bottle adhesive system was applied on etched or unetched deep dentine.Methods. Eighteen class V deep cavity preparations were divided in three groups: group 1-total etching + two coats of single bond (SB) + composite resin (Z-100); group 2-enamel etching + two coats of SB + Z-100, group 3-cavity floor lined with a calcium hydroxide liner (Dycal) + acid-etching of enamel and lateral walls + two coats of SB + Z-100. Two teeth were used as intact control group. After 30 days the teeth were extracted and processed through H and E, Masson's trichrome and Brown and Brenn staining techniques.Results. Moderate inflammatory response, disorganization of pulp tissue, as well as, deposition of thin layer of reactionary dentin were observed in group 1 teeth in which the remaining dentin thickness (RDT) was less than 300 mum. These histological findings appear to be related to long resin tags formation and bonding agent diffusion through dentinal tubules. In group 2, slight inflammatory response was observed only in one tooth in which the RDT was 162 mum. In group 3, all the teeth showed normal histological characteristics which were similar to the intact control group. Presence of bacteria was not correlated with the intensity of pulpal response. The patients reported no symptoms during the experiment. Radiographic evaluation showed no periapical pathology for any of the teeth,Significance and conclusions. Acid-etched deep dentin (RDT less than 300 mum) lined with SB causes more intense pulpal response than unetched deep dentin. Based on the results observed in the present study and the conditions in which it was carried out, we recommend the application of a biocompatible liner before etching deep dentin and applying SB. (C) 2002 Academy of Dental Materials. Published by Elsevier Science Ltd. All rights reserved.