Evaluation of urmiary 8-hydroxydeoxyguanine in healthy Japanese people
Evaluation of urmiary 8-hydroxydeoxyguanine in healthy Japanese people
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DOI：
10.1111/j.1742-7843.2006.pto_217.x
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发表时间：
2006-05-01
影响因子：
3.1
通讯作者：
Ogino, Keiki
中科院分区：
文献类型：
--
作者：
Kimura, Shingo;Yamauchi, Hiroshi;Ogino, Keiki
测定了248名日本健康人的尿中8-羟基脱氧鸟嘌呤（8-OHdG）浓度，并研究了其与生活方式、尿金属元素、血清抗氧化剂和其他血浆或血清因素的相关性。8-OHdG的平均尿浓度为15.2 +/- 5.71 ng/mg肌酐。平均尿8-OHdG在年龄（< 45岁，>= 45岁）、性别、吸烟（无，< 20岁，>= 20岁）和饮酒（无，偶尔，有时和通常）方面无显著差异。此外，多元回归分析显示，尿8-OHdG与尿砷（As）或铬（Cr）之间存在显着相关性，并且前者与铝（Al）和镍（Ni）之间存在相关性趋势。年龄、性别和血浆或血清因素（包括抗氧化剂、脂质过氧化物、HbAlc、BUN和铁）未显示出这种关联。本研究表明，自然暴露于有毒金属元素，如砷，铬，镍可能会影响正常环境管理下的健康人的DNA氧化损伤。因此，测定尿中As、Ni、Cr等金属元素是研究尿中8-OHdG与其他变量关系的前提。
The urinary concentration of 8-hydroxydeoxyguanine (8-OHdG), which is a biomarker of oxidative DNA damage, was measured in 248 healthy Japanese, and its correlations with life style, urinary metal elements, serum antioxidants, and other plasma or serum factors were investigated. The mean urinary concentration of 8-OHdG was 15.2 +/- 5.71 ng/mg creatinine. Mean urinary 8-OHdG was not significantly different in terms of age (< 45, >= 45), gender, smoking (no, < 20, >= 20), and alcohol consumption (no, occasionally, sometimes and usually). Moreover, multiple regression analysis showed a significant association between urinary 8-OHdG and urinary arsenic (As) or chromium (Cr), and a tendency for association between the former and aluminum (Al) and nickel (Ni). Age, gender and plasma or serum factors including antioxidants, lipid peroxide, HbAlc, BUN, and iron did not show such an association. The present study suggests that natural exposure to toxic metal elements such as As, Cr, and Ni may influence oxidative DNA damage in healthy people under usual environmental management. Therefore, the measurement of urinary metals such as As, Ni and Cr is prerequisite for the study of the relationship between urinary 8-OHdG and other variable factors.