Proteolytic Cleavage of the Fusion Protein but Not Membrane Fusion Is Required for Measles Virus-Induced Immunosuppression In Vitro
Proteolytic Cleavage of the Fusion Protein but Not Membrane Fusion Is Required for Measles Virus-Induced Immunosuppression In Vitro
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麻疹病毒诱导的体外免疫抑制需要融合蛋白的蛋白水解切割而不是膜融合
DOI：
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发表时间：
2000
影响因子：
5.4
通讯作者：
S. Schneider‐Schaulies
中科院分区：
文献类型：
--
作者：
A. Weidmann;A. Maisner;W. Garten;Marion Seufert;V. ter meulen;S. Schneider‐Schaulies
ABSTRACT Immunosuppression induced by measles virus (MV) is associated with unresponsiveness of peripheral blood lymphocytes (PBL) to mitogenic stimulation ex vivo and in vitro. In mixed lymphocyte cultures and in an experimental animal model, the expression of the MV glycoproteins on the surface of UV-inactivated MV particles, MV-infected cells, or cells transfected to coexpress the MV fusion (F) and the hemagglutinin (H) proteins was found to be necessary and sufficient for this phenomenon. We now show that MV fusion-inhibitory peptides do not interfere with the induction of immunosuppression in vitro, indicating that MV F-H-mediated fusion is essentially not involved in this process. Proteolytic cleavage of MV F0 protein by cellular proteases, such as furin, into the F1-F2subunits is, however, an absolute requirement, since (i) the inhibitory activity of MV-infected BJAB cells was significantly impaired in the presence of a furin-inhibitory peptide and (ii) cells expressing or viruses containing uncleaved F0 proteins revealed a strongly reduced inhibitory activity which was improved following trypsin treatment. The low inhibitory activity of effector structures containing mainly F0 proteins was not due to an impaired F0-H interaction, since both surface expression and cocapping efficiencies were similar to those found with the authentic MV F and H proteins. These results indicate that the fusogenic activity of the MV F-H complexes can be uncoupled from their immunosuppressive activity and that the immunosuppressive domains of these proteins are exposed only after proteolytic activation of the MV F0protein.
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使用生物测定、NMR 和分子模型分析副粘病毒 (NDV) 融合的肽抑制剂。
DOI：
10.1006/viro.1997.8834
发表时间：
1997
期刊：
Virology.
影响因子：
--
作者：
Young,JK;Hicks,RP;Wright,GE;Morrison,TG
通讯作者：
Morrison,TG
亮氨酸残基在副粘病毒（NDV）融合亮氨酸拉链肽抑制剂的结构和功能中的作用。
DOI：
10.1006/viro.1998.9044
发表时间：
1998
期刊：
Virology.
影响因子：
--
作者：
Young,JK;Hicks,RP;Wright,GE;Morrison,TG
通讯作者：
Morrison,TG
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影响因子：
3.7
作者：
Yanagi,Y;Cubitt,BA;Oldstone,MB
通讯作者：
Oldstone,MB
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DOI：
--
发表时间：
1988
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
McChesney,MB;Altman,A;Oldstone,MB
通讯作者：
Oldstone,MB
DOI：
10.1073/pnas.91.26.12676
发表时间：
1994-12-20
影响因子：
11.1
作者：
WILD, C;DUBAY, JW;MATTHEWS, T
通讯作者：
MATTHEWS, T