Osteoclast development in marrow cultured in calvaria-conditioned media.
Osteoclast development in marrow cultured in calvaria-conditioned media.
复制标题
在颅盖条件培养基中培养的骨髓中破骨细胞的发育。
DOI：
10.1016/0012-1606(88)90198-4
复制
发表时间：
1988
影响因子：
2.7
通讯作者：
Osdoby,P
中科院分区：
文献类型：
--
作者：
Oursler,MJ;Osdoby,P
负责招募和破骨细胞（OC）从其单核前体分化的精确信号知之甚少。骨髓单个核细胞，一种公认的OC前体来源，在培养中融合，形成多核细胞。这些细胞，虽然类似于OC，但在细胞表面形态上不同于破骨细胞，并且不被OC特异性单克隆抗体识别。我们已经使用了破骨细胞特异性膜表位的单克隆抗体121 F指定的描绘OC从骨髓来源的巨细胞（MAGC）的表达。在这份报告中，我们描述了一系列的实验设计，以更好地确定骨环境中的破骨细胞分化过程中的作用。将来自幼雏的无骨膜颅骨或其条件培养基与贴壁第1天培养的骨髓细胞组合。通过ELISA监测OC标志物表达的时间过程，并研究对活骨和PTH的需求。新鲜分离的骨髓、MAGC和颅骨没有OC表达。在与活骨或活骨条件培养基共接种4天后，在培养的MAGC中产生抗原表达。PTH的存在下，在共培养物或条件培养基从PTH处理的头盖骨没有显着改变的表达水平。这些数据表明，活骨是，在一定程度上，负责从单核前体细胞的破骨细胞的生产。
The precise signals responsible for recruitment and differentiation of osteoclasts (OCs) from their mononuclear precursors are poorly understood. Marrow mononuclear cells, a reputed source of OC precursors, fuse in culture, forming multinucleated cells. These cells, although similar to OCs, differ from osteoclasts in cell-surface morphology and are not recognized by an OC-specific monoclonal antibody. We have used the expression of an osteoclast-specific membrane epitope designated by monoclonal antibody 121F to delineate OCs from marrow-derived giant cells (MAGC). In this report we describe a series of experiments designed to better define the role of the bone environment in the osteoclast differentiation process. Periosteum-free calvariae from hatchling chicks or their conditioned media were combined with adherent Day 1 cultured marrow cells. The time course of OC marker expression was monitored by ELISA and the requirement for live bone and PTH was investigated. Freshly isolated marrow, MAGC, and calvariae were devoid of OC expression. Antigen expression developed in cultured MAGC after 4 days of coplating with either live bone or live bone-conditioned media. The presence of PTH in the cocultures or conditioned media from PTH-treated calvariae did not significantly alter the level of expression. These data indicate that live bone is, in part, responsible for the production of osteoclasts from mononuclear precursors.