Electrostatic ligand coatings of nanoparticles enable ligand-specific gene delivery to human primary cells
Electrostatic ligand coatings of nanoparticles enable ligand-specific gene delivery to human primary cells
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DOI：
10.1021/nl062395b
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发表时间：
2007-04-01
期刊：
影响因子：
10.8
通讯作者：
Anderson, Daniel G.
中科院分区：
文献类型：
--
作者：
Green, Jordan J.;Chiu, Eugene;Anderson, Daniel G.
开发了一种涂覆聚合物/DNA 纳米颗粒的通用方法。研究发现，肽包覆的纳米颗粒具有良好的生物物理特性，包括粒径小、接近中性的 zeta 电位以及在血清中的稳定性。在适当的配制条件（包括接近中性电荷比）下，包被的纳米粒子能够将配体特异性基因有效地递送至含血清培养基中的人原代内皮细胞。由于这种纳米颗粒药物递送系统具有高效、基于配体的特异性、生物可降解性和低细胞毒性，因此它可能在多种临床应用中具有潜在用途。
A general method of coating polymer/DNA nanoparticles was developed. Peptide coated nanoparticles were found to have favorable biophysical characteristics including small particle size, near-neutral zeta potential, and stability in serum. At appropriate formulation conditions including near-neutral charge ratio, the coated nanoparticles enabled effective ligand-specific gene delivery to human primary endothelial cells in serum-containing media. As this nanoparticulate drug delivery system has high efficacy, ligand-based specificity, biodegradability, and low cytotoxicity, it may be potentially useful in several clinical applications.