Monoalkylation of DNA by reductively activated FR66979.
Monoalkylation of DNA by reductively activated FR66979.
复制标题
还原激活的 FR66979 对 DNA 进行单烷基化。
DOI：
10.1016/s0968-0896(99)00270-9
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发表时间：
2000
影响因子：
3.5
通讯作者：
Hopkins,PB
中科院分区：
文献类型：
--
作者：
Paz,MM;Sigurdsson,ST;Hopkins,PB
The antitumor antibiotic FR66979 has previously been shown to form interstrand cross-links in duplex DNA at the sequence [5′-d(CG)]2, linking the exocyclic amino groups (N2) of deoxyguanosine (dG) residues. During the reaction of reductively activated FR66979 with DNA, products are formed which have electrophoretic mobility in denaturing polyacrylamide gels which is intermediate between that of unmodified and interstrand cross-linked DNA. We show here that these products are monoadducts between FR66979 and DNA and provide strong evidence for the site of alkylation being N2 of dG. Moreover, the sequence selectivity of monoalkylation reactions between FR66979 and DNA containing either 5′-d(CG)·5′-d(CI) or [5′-d(CG)]2was observed to be ca. 5-fold less than for the related antitumor antibiotic mitomycin C (MC). The mechanistic implications of this result are discussed. Furthermore, it was demonstrated that contrary to a previous report, FR66979 requires DNA to be in duplex form for efficient monoadduct formation.
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FR66979 需要还原激活才能有效地交联 DNA
DOI：
--
发表时间：
1994
期刊：
影响因子：
--
作者：
Huifang Huang;S. Rajski;Robert M. Williams;P. B. Hopkins
通讯作者：
P. B. Hopkins
FR66979 和 FR900482 的 DNA-DNA 链间交联：还原活化过程中需要金属离子
DOI：
--
发表时间：
1997
期刊：
影响因子：
--
作者：
M. Paz;P. B. Hopkins
通讯作者：
P. B. Hopkins
影响因子：
56.9
作者：
TOMASZ, M;LIPMAN, R;NAKANISHI, K
通讯作者：
NAKANISHI, K
影响因子：
15
作者：
HUANG, HF;PRATUM, TK;HOPKINS, PB
通讯作者：
HOPKINS, PB
FR900482，丝裂霉素 C 的近亲，利用基于丝裂霉素的 DNA 交联。
影响因子：
--
作者：
Williams,RM;Rajski,SR;Rollins,SB
通讯作者：
Rollins,SB