Evaluate the bisphenol A-induced redox state in cells, zebrafish and in vivo with a hydrogen peroxide turn-on fluorescent probe.
Evaluate the bisphenol A-induced redox state in cells, zebrafish and in vivo with a hydrogen peroxide turn-on fluorescent probe.
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DOI：
10.1016/j.jhazmat.2021.127425
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发表时间：
2021-10
影响因子：
13.6
通讯作者：
Yan Huang;Lei Yu;Pengpeng Lu;Yinghui Wei;Lili Fu;J. Hou;Yunqing Wang;Xiaoyan Wang;Lingxin Chen
中科院分区：
文献类型：
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作者：
Yan Huang;Lei Yu;Pengpeng Lu;Yinghui Wei;Lili Fu;J. Hou;Yunqing Wang;Xiaoyan Wang;Lingxin Chen
过氧化氢（H2O2）是一种重要的活性氧，在生物系统的氧化还原平衡和各种疾病的生理和病理过程中起着重要作用。由于H2O2是一种内源性活性分子，其含量的波动不仅受生物系统本身状态的影响，而且易受外界环境中双酚A（BPA，一种典型的雌激素环境污染物）的影响。本文合成了近红外荧光探针Cy-NOH2（λ em = 750 nm），用于检测BPA引起的细胞和生物体内H2O2含量的波动。用Cy-NOH_2探针检测内源性H_2O_2具有较高的灵敏度和良好的选择性。此外，该探针还能检测到不同浓度BPA诱导的H2O2的表达。用不同浓度的BPA诱导斑马鱼和小鼠模型，随着BPA浓度的增加，斑马鱼和小鼠肝脏中H2O2含量均呈显著增加趋势。本研究揭示Cy-NOH2探针可作为监测BPA影响下体内氧化还原状态的有效工具，为阐明BPA在多种生理病理过程中的作用机制提供了依据。
Hydrogen peroxide (H2O2) is an important active oxygen species that plays a major role in redox balance and in physiological and pathological processes of various diseases of biological systems. As H2O2is an endogenous active molecule, fluctuations in H2O2content are not only affected by the state of biological system itself but also easily affected by Bisphenol A (BPA, a typical estrogenic environmental pollutant) in the external environment. Here, the near-infrared fluorescent probeCy-NOH2(λem= 750 nm) as a tool was synthesized to detect fluctuations in H2O2content in cells and organisms induced by BPA. High sensitivity and excellent selectivity were found when the probeCy-NOH2was used to monitor endogenous H2O2in vitro. In addition, the expression of H2O2induced by different concentrations of BPA was able to be detected by the probe. Zebrafish and mice models were induced with different concentrations of BPA, and the H2O2content showed significant increasing trends in zebrafish and livers of mice with increasing BPA concentrations. This study reveals that the probeCy-NOH2can be used as an effective tool to monitor the redox state in vivo under the influence of BPA, which provides a basis for clarifying the mechanisms of BPA in a variety of physiological and pathological processes.