AXONAL GUIDANCE MUTANTS OF CAENORHABDITIS-ELEGANS IDENTIFIED BY FILLING SENSORY NEURONS WITH FLUORESCEIN DYES
AXONAL GUIDANCE MUTANTS OF CAENORHABDITIS-ELEGANS IDENTIFIED BY FILLING SENSORY NEURONS WITH FLUORESCEIN DYES
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DOI：
10.1016/0012-1606(85)90443-9
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发表时间：
1985-01-01
影响因子：
2.7
通讯作者：
PERKINS, LA
中科院分区：
文献类型：
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作者：
HEDGECOCK, EM;CULOTTI, JG;PERKINS, LA
秀丽隐杆线虫的八对化学感受神经元摄取通过化学感受器官进入的荧光素染料。这些是两栖类神经元ADF、ASH、ASI、ASJ、ASK和ADL以及phasmid神经元PHA和PHB。当充满染料时，这些神经元的突起和细胞体可以在活体动物中通过荧光显微镜进行检查。利用这种技术，我们已经确定了五个基因，unc-33，unc-44，unc-51，unc-76和unc-106，影响的两栖类和phasmid轴突的生长。发现这些基因也影响机械感觉PDE神经元的轴突。unc-33突变特异性影响神经元微管。这种突变体的感觉树突有过多的微管。而且，这些微管中的许多是直径异常的，并且一些形成钩状或多个微管。
Eight pairs of chemosensory neurons in Caenorhabditis elegans take up fluorescein dyes entering through the chemosensory organs. These are amphid neurons ADF, ASH, ASI, ASJ, ASK, and ADL and phasmid neurons PHA and PHB. When filled with dye, the processes and cell bodies of these neurons can be examined in live animals by fluorescence microscopy. Using this technique, we have identified five genes, unc-33, unc-44, unc-51, unc-76, and unc-106, that affect the growth of the amphid and phasmid axons. These genes were found to affect the axons of the mechanosensory PDE neurons as well. The unc-33 mutation specifically affects neuronal microtubules. Sensory dendrites in this mutant have a superabundance of microtubules. Moreover, many of these microtubules are abnormal in diameter, and some form hooks or multiple tubules.