DISTRIBUTION OF ACTIN AND TUBULIN IN CELLS AND IN GLYCERINATED CELL MODELS AFTER TREATMENT WITH CYTOCHALASIN-B (CB)
DISTRIBUTION OF ACTIN AND TUBULIN IN CELLS AND IN GLYCERINATED CELL MODELS AFTER TREATMENT WITH CYTOCHALASIN-B (CB)
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DOI：
10.1016/0014-4827(76)90044-6
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发表时间：
1976-01-01
影响因子：
3.7
通讯作者：
FRANKE, WW
中科院分区：
文献类型：
--
作者：
WEBER, K;RATHKE, PC;FRANKE, WW
使用肌动蛋白、微管蛋白和原肌球蛋白特异性抗体，通过EM和免疫荧光显微镜研究了添加细胞松弛素B（CB）前后培养细胞[小鼠成纤维细胞3T3细胞、大鼠乳腺成纤维细胞和大鼠乳腺癌]中微丝和微管的组织。CB诱导正常微丝束的快速解体。免疫荧光显微镜下可见星形的肌动蛋白和原肌球蛋白斑块，电镜下可见密集的凝聚微丝聚集体。CB处理不会改变微管的完整性。与正常细胞相比，向甘油化的细胞中加入CB不会导致微丝束的紊乱。CB处理的甘油化模型在加入ATP后仍然可以收缩。因此，CB诱导的微丝束重排只发生在体内，而不是在甘油化的细胞收缩模型。
The organization of microfilaments and microtubules in cultured cells [mouse fibroblast 3T3 cells, rat mammary fibroblasts rat mammary carcinoma] before and after the addition of cytochalasin B (CB) was studied both by EM and immunofluorescence microscopy using antibodies specific for actin, tubulin and tropomyosin. CB induces a rapid disorganization of normal microfilament bundles. Star-like patches of actin and tropomyosin are visualized in immunofluorescence microscopy and dense aggregates of condensed microfilaments are seen in EM. The integrity of the microtubules is not changed by CB treatment. Addition of CB to glycerinated cells, in contrast to normal cells, does not result in the disorganizati-n of microfilament bundles. CB-treated glycerinated models can still contract upon addition of ATP. Thus the CB-induced rearrangement of microfilament bundles occurs only in vivo and not in glycerinated cell contractility models.