REMOVAL OF ENDOTOXINS BY AFFINITY SORBENTS
REMOVAL OF ENDOTOXINS BY AFFINITY SORBENTS
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DOI：
10.1016/0021-9673(95)00126-8
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发表时间：
1995-09-08
影响因子：
4.1
通讯作者：
HILBECK, O
中科院分区：
文献类型：
--
作者：
ANSPACH, FB;HILBECK, O
组氨酸、组胺和多粘菌素B亲和吸附剂用于去除大肠杆菌来源的内毒素。并与多聚L赖氨酸琼脂糖凝胶和二乙醇胺琼脂糖凝胶进行比较。所有的吸附剂都能将大肠杆菌培养滤液中的内毒素浓度降低到可耐受的水平。然而，它们的效果并不比阴离子交换器高，后者的清除率高达15000。蛋白质溶液中内毒素的去除依赖于所需蛋白质的净电荷。溶菌酶作为正电蛋白的模型可促进内毒素的清除。相比之下，只有较低的初始污染水平(
Histidine, histamine and polymyxin B affinity sorbents were employed for the removal of Escherichia coli-derived endotoxins. Their effectiveness was compared with those of poly-L-lysine-Sepharose and DEAE-Sepharose. All sorbents reduced the concentration of endotoxins from an E. coli culture filtrate to tolerable levels. However, their effectiveness was not higher than that of the anion exchanger, which displayed clearance rates of up to 15 000. Endotoxin removal from protein solutions depended on the net charge of the desired protein. Lysozyme as a model for positively charged proteins enhanced endotoxin removal. In contrast, only low initial contamination levels (