Factors affecting the permissiveness of porcine alveolar macrophages for porcine reproductive and respiratory syndrome virus
Factors affecting the permissiveness of porcine alveolar macrophages for porcine reproductive and respiratory syndrome virus
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DOI：
10.1007/s00705-008-0271-y
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发表时间：
2009-01-01
影响因子：
2.7
通讯作者：
Wyatt, C. R.
中科院分区：
文献类型：
--
作者：
Gaudreault, N.;Rowland, R. R. R.;Wyatt, C. R.
将PRRSV感染和未感染的猪肺泡巨噬细胞置于培养中，感染PRRSV实验室菌株SD-23983。随着培养时间的延长，允许度增加，感染猪的巨噬细胞可被重复感染。添加mRNA合成抑制剂放线菌素D可阻断感染。干扰素- γ减少了培养物中的感染，而肿瘤坏死因子- α或白细胞介素(IL)-10的加入对容纳性没有影响。IL-4使受感染细胞的百分比略有增加，但病毒产量没有明显增加。这些结果表明，培养中允许prrsv的细胞群体来自非允许的前体群体，并依赖于新的mRNA合成。
Alveolar macrophages from PRRSV-infected and na < ve pigs were placed into culture and infected with PRRSV laboratory strain SD-23983. Permissiveness increased with time in culture, and macrophages from infected pigs could be superinfected. Addition of actinomycin D, an inhibitor of mRNA synthesis, blocked infection. Interferon-gamma reduced infection in cultures, while the addition of tumor necrosis factor-alpha or interleukin (IL)-10 did not affect permissiveness. IL-4 produced a marginal increase in the percentage of infected cells, but without a detectable increase in virus yield. These results suggest that the PRRSV-permissive population of cells in culture arises from a non-permissive precursor population and depends on new mRNA synthesis.