Assessing Tissue-Specific Autophagy Flux in Adult Caenorhabditis elegans.
Assessing Tissue-Specific Autophagy Flux in Adult Caenorhabditis elegans.
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DOI：
10.1007/978-1-0716-0592-9_17
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发表时间：
2020
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自噬的细胞循环过程对于生存、发展和动态平衡是必不可少的。自噬在衰老中也扮演着重要的角色，在许多物种中都与长寿有关，包括线虫线虫。研究自噬在线虫衰老过程中的生理作用需要对自噬进行时空分析。在这里，我们描述了一种方法，通过使用荧光标记的Atg8/LGG-1报告来定量自噬小泡池，以评估线虫多个组织中的自噬通量。这一方法揭示了线虫在衰老过程中不同细胞类型的自噬活性不同。
The cellular recycling process of autophagy is essential for survival, development, and homeostasis. Autophagy also plays an important role in aging and has been linked to longevity in many species, including the nematode C. elegans. Study of the physiological roles of autophagy during C. elegans aging requires methods for the spatiotemporal analysis of autophagy. Here we describe a method for assessing autophagic flux in multiple tissues of C. elegans by quantifying the pool of autophagic vesicles using fluorescently labelled Atg8/LGG-1 reporters upon autophagy inhibition using bafilomycin A1 (BafA). This methodology has revealed that autophagic activity varies in different cell types of C. elegans during aging.