Identification of quantitative trait loci associated with germination using chromosome segment substitution lines of rice (Oryza sativa L.)
Identification of quantitative trait loci associated with germination using chromosome segment substitution lines of rice (Oryza sativa L.)
复制标题
使用水稻染色体片段替换系鉴定与发芽相关的数量性状基因座（Oryza sativa L.）
DOI：
10.1007/s00122-011-1593-9
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发表时间：
2011-08-01
影响因子：
5.4
通讯作者：
Yu, Sibin
中科院分区：
文献类型：
--
作者：
Li, Min;Sun, Penglin;Yu, Sibin
在不同的环境条件下，种子的快速和均匀发芽是大多数作物的理想特性，如水稻、小麦和玉米。然而，在发芽率变异的遗传基础还不是很清楚。本研究以籼稻品种珍山97为统一遗传背景，利用143个染色体片段代换系（CSSL）对发芽率的数量性状位点（QTL）进行了定位。9株csl的发芽率显著低于镇山97。在2、5、6和10号染色体上鉴定出4个与萌发相关的QTL，这些等位基因均降低了粳稻的发芽率。利用cssl衍生的F2群体，确认了2号染色体上的一个主QTL (qGR2)，并将其划分为10.4 kb的区间，包含3个候选基因，其中OsMADS29仅在种子中优先表达。这些结果将有助于克隆影响发芽率的主要基因，并对发芽的遗传基础提供深入了解。
Rapid and uniform seed germination under diverse environmental conditions is a desirable characteristic for most crop plants, such as rice, wheat, and maize. However, the genetic base of the variations in the rate of germination is not well understood. In this study, quantitative trait loci (QTL) for germination rate were mapped with a set of 143 chromosome segment substitution lines (CSSL) each contains a small genomic fragment from a japonica variety Nipponbare in the uniform genetic background of an indica variety Zhenshan97. Nine CSSL showed significantly lower germination rate than that in Zhenshan97. Four germination-related QTL were identified located on chromosomes 2, 5, 6 and 10, at which all japonica alleles decreased germination rate. By using the CSSL-derived F2 population, a major QTL (qGR2) on chromosome 2 was confirmed, and delimited to a 10.4 kb interval containing three putative candidate genes, of which OsMADS29 was only expressed preferentially in the seed. These results would facilitate cloning of the major gene that affects germination rate, and provide an insight into the genetic basis of germination.