Interspecies linkage analysis of mo, a Bombyx mori locus associated with mosaicism and gynandromorphism
Interspecies linkage analysis of mo, a Bombyx mori locus associated with mosaicism and gynandromorphism
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mo（与嵌合体和雌雄同体相关的家蚕基因座）的种间连锁分析
DOI：
10.1007/s10709-012-9634-0
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发表时间：
2012
期刊：
影响因子：
1.5
通讯作者：
Fujii Tsuguru
中科院分区：
文献类型：
--
作者：
Arimasa Matsumoto;Laurean Ilies;Eiichi Nakamura;松本有正・NORINDER Jakob・吉戒直彦・中村栄一;Daimon Takaaki;Hara Kahori;Fujii Tsuguru
西莫（遗传马赛克）突变是家蚕最著名和有趣的突变之一。由于极体未消除和随后的双受精事件，雌性纯合子产生马赛克和雌雄同体的后代，而与交配的雄性的基因型无关。虽然该基因于1927年首次报道，但由于突变不稳定且对遗传背景敏感，该基因座尚未被定位到连锁群。在本研究中，使用基于pcr的标记对单核苷酸多态性连锁图谱进行了mox的连锁分析。中国家蚕被用来作为b的交配对象。morimostrain，因为更容易识别b。moriandB。mandarinathan withinB。moristrains。令人惊讶的是，我们在所有12只B1（第一代回交）飞蛾中发现了两个纯合连锁群（LGs）。结果表明+mois位于b的M染色体上。它对应于b的两个连锁群。mori， lg14和lg27。基于进一步的连锁分析，使用b。moras是一个交配伙伴，moas被映射到lg14。此外，我们发现lg17上的一个基因可以修饰气氛活性。
Themo(hereditary mosaic) mutation is one of the most famous and interesting mutations of the silkworm,Bombyx mori. Females homozygous formogenerate mosaic and gynandromorphic offspring due to non-elimination of polar bodies and subsequent double fertilization events, irrespective of the genotype of the mated males. Althoughmowas first reported in 1927, the locus has not been mapped to linkage groups, as the mutation is unstable and appears to be sensitive to genetic background. In this study, linkage analysis ofmowas performed using PCR-based markers on single nucleotide polymorphism linkage maps.Bombyx mandarinawas used as the mating partner for theB. morimostrain, as it is easier to identify polymorphic markers betweenB. moriandB. mandarinathan withinB. moristrains. Surprisingly, we identified two homozygous linkage groups (LGs) in all of the 12 B1(first backcross generation) moths that had deposited mosaic eggs. It was revealed that +mois located on the M chromosome ofB. mandarina,which corresponds to two linkage groups ofB. mori,LG 14 and 27. Based on further linkage analysis usingB. morias a mating partner,mowas mapped to LG 14. Additionally, we found thatmoactivity could be modified by a gene(s) on LG 17.