Functional expression of a Δ12 fatty acid desaturase gene from spinach in transgenic pigs
Functional expression of a Δ12 fatty acid desaturase gene from spinach in transgenic pigs
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DOI：
10.1073/pnas.0308111101
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发表时间：
2004-04-27
影响因子：
11.1
通讯作者：
Iritani, A
中科院分区：
文献类型：
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作者：
Saeki, K;Matsumoto, K;Iritani, A
亚油酸（18：2n-6）和α-亚麻酸（18：3 n-3）是哺乳动物营养所必需的多不饱和脂肪酸，因为哺乳动物缺乏合成Δ 12（n-6）和n-3脂肪酸所需的去饱和酶。许多植物可以合成这些脂肪酸，因此，为了研究植物去饱和酶在哺乳动物中的作用，我们产生了转基因猪，这些猪携带来自菠菜的Delta 12脂肪酸去饱和酶的脂肪酸去饱和2基因。转基因猪脂肪细胞在体外分化的亚油酸（18：2n-6）水平比野生型猪高10倍。此外，转基因猪的白色脂肪组织中亚油酸（18：2n-6）含量比野生型猪高出近20%。这些结果证明了脂肪酸去饱和酶的植物基因在哺乳动物中的功能性表达，从而打开了使用脂肪酸去饱和酶的植物基因通过转基因技术改变来自家畜的产品的脂肪酸组成的可能性。
Linoleic acid (18:2n-6) and a-linolenic acid (18:3n-3) are polyunsaturated fatty acids that are essential for mammalian nutrition, because mammals lack the desaturases required for synthesis of Delta12 (n-6) and n-3 fatty acids. Many plants can synthesize these fatty acids and, therefore, to examine the effects of a plant desaturase in mammals, we generated transgenic pigs that carried the fatty acid desaturation 2 gene for a Delta12 fatty acid desaturase from spinach. Levels of linoleic acid (18:2n-6) in adipocytes that had differentiated in vitro from cells derived from the transgenic pigs were approximate to10 times higher than those from wild-type pigs. In addition, the white adipose tissue of transgenic pigs contained approximate to20% more linoleic acid (18:2n-6) than that of wild-type pigs. These results demonstrate the functional expression of a plant gene for a fatty acid desaturase in mammals, opening up the possibility of modifying the fatty acid composition of products from domestic animals by transgenic technology, using plant genes for fatty acid desaturases.