RNA binding fragments from nucleolin contain the ribonucleoprotein consensus sequence.
RNA binding fragments from nucleolin contain the ribonucleoprotein consensus sequence.
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DOI：
10.1016/s0021-9258(18)60904-4
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发表时间：
1987-08
期刊：
影响因子：
--
通讯作者：
B. Bugler;H. Bourbon;B. Lapeyre;M. O. Wallace;J. Chang;F. Amalric;M. Olson
中科院分区：
文献类型：
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作者：
B. Bugler;H. Bourbon;B. Lapeyre;M. O. Wallace;J. Chang;F. Amalric;M. Olson
核仁蛋白(C23或100 kDa)是一种主要的核仁磷酸蛋白，其一级结构最近被确定(Lapeyre，B.，Bourbon，H.和AMalric，F.(1987)过程。娜塔莉。阿卡德。SCI。美国A.84,1472-1476)，并发现与胸前RNA有关(Herrera，A.H.和Olson，M.O.J.(1986)生物化学25,6258-6263)。为了确定该分子的核糖体核糖核酸结合区，用“蛋白质印迹”技术检测了18个S和28个S核糖体核糖体核糖核酸的结合。表观分子质量为13、33和47 kDa的片段与RNA结合，对18 S或28 S没有偏好。通过蛋白质测序，这些片段定位在分子的三分之二的羧基末端。在核仁序列中搜索在其他RNA结合蛋白中发现的核糖核蛋白共同序列。在残基285和629之间的RNA结合区，在80-90个残基重复序列中发现了密切相关的11个残基序列的四个拷贝。这些结果表明，几种蛋白质都利用了一个高度保守的结构来与不同类别的RNA结合。
Nucleolin (C23 or 100 kDa) is a major nucleolar phosphoprotein whose primary structure has recently been determined (Lapeyre, B., Bourbon, H., and Amalric, F. (1987) Proc. Natl. Acad. Sci. U. S. A. 84, 1472-1476) and found to be associated with preribosomal RNA (Herrera, A. H., and Olson, M. O. J. (1986) Biochemistry 25, 6258-6263). To identify the RNA binding region of the molecule, cyanogen bromide fragments were tested for binding of 18 S and 28 S ribosomal RNA by a “Western blotting” technique. Fragments with apparent molecular masses of 13, 33, and 47 kDa bound RNA with no preference for either 18 S or 28 S RNA. By protein sequencing, these fragments were localized in the carboxyl-terminal two-thirds of the molecule. The nucleolin sequence was searched for the ribonucleoprotein consensus sequence found in other RNA binding proteins. Four copies of a closely related 11-residue sequence were found within 80-90 residue repeats in the RNA binding region between residues 285 and 629. These results suggest that a highly conserved structure for the binding of different classes of RNA is utilized by several proteins.