The effect of intracellular pH on contractile function of intact, single fibres of mouse muscle declines with increasing temperature.
The effect of intracellular pH on contractile function of intact, single fibres of mouse muscle declines with increasing temperature.
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细胞内 pH 值对完整的小鼠肌肉单纤维收缩功能的影响随着温度的升高而减弱。
DOI：
10.1113/jphysiol.1997.sp022009
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
J. Lännergren
中科院分区：
文献类型：
--
作者：
H. Westerblad;J. Bruton;J. Lännergren
1.研究了改变细胞内pH(Phi)对小鼠骨骼肌单纤维在12℃、22℃和32℃时等长收缩和收缩速度的影响。PHI的变化是通过将纤维暴露在不同二氧化碳浓度的溶液中而获得的。2.在控制条件下(5%CO2)，PHI(用羧基SNARF-1测定)在各个温度下都比中性水多出约0.3个pH单位的碱性。30%的二氧化碳产生了约0.5pH单位的酸化，而0%的二氧化碳产生了类似大小的碱化。3.在酸化纤维中，强直力在12℃时降低了28%，而在32℃时仅降低了10%，随着碱化程度的增加，强直力随温度的升高而降低。酸化引起明显的松弛减慢，并且这种减慢随着温度的升高而变小。4.在12℃时，酸化使最大缩短速度(V0)降低了近20%，而在32℃时，酸化对V0的影响不显著。5.综上所述，随着温度的升高，pHi对哺乳动物肌肉收缩的影响明显减弱。因此，酸化对力量产生的直接抑制不是生理温度下肌肉疲劳的主要因素。
1. The effect of altered intracellular pH (pHi) on isometric contractions and shortening velocity at 12, 22 and 32 degrees C was studied in intact, single fibres of mouse skeletal muscle. Changes in pHi were obtained by exposing fibres to solutions with different CO2 concentrations. 2. Under control conditions (5% CO2), pHi (measured with carboxy SNARF‐1) was about 0.3 pH units more alkaline than neutral water at each temperature. An acidification of about 0.5 pH units was produced by 30% CO2 and an alkalinization of similar size by 0% CO2. 3. In acidified fibres tetanic force was reduced by 28% at 12 degrees C but only by 10% at 32 degrees C. The force increase with alkalinization showed a similar reduction with increasing temperature. Acidification caused a marked slowing of relaxation and this slowing became less with increasing temperature. 4. Acidification reduced the maximum shortening velocity (V0) by almost 20% at 12 degrees C, but had no significant effect at 32 degrees C. Alkalinization had no significant effect on V0 at any temperature. 5. In conclusion, the effect of pHi on contraction of mammalian muscle declines markedly with increasing temperature. Thus, the direct inhibition of force production by acidification is not a major factor in muscle fatigue at physiological temperatures.
影响因子：
5.5
作者：
PATE, E;BHIMANI, M;COOKE, R
通讯作者：
COOKE, R
高碳酸血症和磷酸浓度增加不会降低猫骨骼肌的力量。
DOI：
10.1152/ajpcell.1991.260.4.c805
发表时间：
1991
期刊：
The American journal of physiology
影响因子：
--
作者：
Adams,GR;Fisher,MJ;Meyer,RA
通讯作者：
Meyer,RA
活体和剥皮青蛙肌肉纤维的最大缩短速度。
DOI：
10.1113/jphysiol.1986.sp015929
发表时间：
1986
期刊：
The Journal of physiology
影响因子：
--
作者：
Julian,FJ;Rome,LC;Stephenson,DG;Striz,S
通讯作者：
Striz,S