SOME MORPHOLOGICAL CHARACTERS OF RICE DWARF VIRUS
SOME MORPHOLOGICAL CHARACTERS OF RICE DWARF VIRUS
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DOI：
10.1016/0042-6822(62)90005-3
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发表时间：
1962-01-01
期刊：
影响因子：
3.7
通讯作者：
SHIKATA, E
中科院分区：
文献类型：
--
作者：
FUKUSHI, T;KIMURA, I;SHIKATA, E
从水稻病叶中提取的水稻矮缩病毒制剂中，一直存在大小和形状均一的小颗粒。或侵染性叶蝉的身体。这些颗粒呈球形或多面体，直径约70米[亩]，并有中央暗区，直径40-50米，周围有相对透明的区域和外膜。类似的颗粒存在于水稻病叶褪绿部分的细胞质中，以及感染叶蝉的脂肪体、血液、肠道上皮、唾液腺和马氏管中。在受感染的昆虫和植物组织的细胞中，这些颗粒经常以晶体阵列的形式出现。所有获得的证据似乎都证明了这些颗粒是水稻矮缩病的致病病毒的结论是正确的。值得注意的是，这似乎是通过电子显微镜在媒介的切片细胞内展示叶蝉传播病毒的第一个记录实例。
Small particles uniform in size and shape have been consistently found in preparations of rice dwarf virus extracted from affected leaves of rice plants. Oryza sativa L., or bodies of infective leafhoppers, Nephotettix cincticeps Uhl. These particles are spherical or polyhedral in shape, approximately 70 m[mu] in diameter, and possess central dark areas, 40-50 m[mu] in diameter, which are surrounded by relatively transparent regions and outer membranes. Similar particles have been shown to exist in the cytoplasm of cells in the chlorotic portions of diseases leaves of rice plants as well as in cells of the fat body, blood, intestinal epithelium, salivary glands, and Malpighian tubules of infective leafhoppers. The particles are frequently seen in crystalline array in the cells of the infected insect and plant tissues. All the evidence secured seems to justify the conclusion that these particles are the causative virus of the rice dwarf disease. It is worthy of note that this seems to be the first recorded instance of the demonstration of a leafhopper-borne virus within sectioned cells of the vector by the electron microscope.