T-cell-receptor gene usage of Actinobacillus actinomycetemcomitans-reactive periodontal CD4+ T cells from localized juvenile periodontitis patients and human peripheral blood leukocyte-reconstituted NOD/SCID mice.
T-cell-receptor gene usage of Actinobacillus actinomycetemcomitans-reactive periodontal CD4+ T cells from localized juvenile periodontitis patients and human peripheral blood leukocyte-reconstituted NOD/SCID mice.
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来自局部幼年牙周炎患者和人外周血白细胞重组 NOD/SCID 小鼠的反应性牙周 CD4 T 细胞的 T 细胞受体基因使用。
DOI：
10.1034/j.1600-0765.2002.01006.x
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发表时间：
2002
影响因子：
3.5
通讯作者：
Teng,Yen-TungA
中科院分区：
文献类型：
--
作者：
Gao,Xuijuan;Teng,Yen-TungA
通过定量ofActinobacillus actinomycetemcomitans‐reactive分析，研究了4例伴放线菌感染的局限性青少年牙周炎(LJP)患者、4组接种 放线菌的NOD/SCID小鼠和4例LJP患者和2例健康对照组的牙周CD+T细胞受体(TCR)的V基因和V基因的使用情况。结果表明：(1)牙周α+T细胞所使用的TcR基因大部分(Vβ占82.5%，V CD4占91.1%)。接种放线菌伴生菌的NOD/SCID小鼠与LJP患者局部牙周T细胞使用的NOD/SCID小鼠有重叠之处，(Ii)虽然A. 放线菌伴生放线菌-反应牙周CD4+TCR谱系相对广泛，但有几个主导基因在LJP患者中共享，这表明共同识别的抗原或表位数量有限，(Iii)A. 放线菌伴随菌群可能缺乏超抗原特性。这些结果表明，在人 -NOD/SCID小鼠中建立的与放线菌伴生菌相关的人CD+T细胞谱系为研究牙周组织中免疫-寄生虫相互作用的特定方面提供了一种有用的方法。
We investigated the variable Vα and Vβ gene usage ofActinobacillus actinomycetemcomitans‐reactive periodontal CD4+T cell receptors (TCR) from: (i) fourA. actinomycetemcomitans‐infected localized juvenile periodontitis (LJP) patients, (ii) four groups ofA. actinomycetemcomitans‐inoculated NOD/SCID mice engrafted with individual LJP‐derived HuPBL and (iii) HuPBL samples of four LJP patients and two healthy control subjects, by quantitative PCR analyses. The results show that: (i) the majority of the TCR genes (82.5% of Vα and 91.1% of Vβ) used by periodontal CD4+T cells inA. actinomycetemcomitans‐inoculated HuPBL‐engrafted NOD/SCID mice overlap with those used by local periodontal T cells in LJP patients, (ii) althoughA. actinomycetemcomitans‐reactive periodontal CD4+TCR repertoire is relatively widespread, there are a few dominant genes shared by the LJP patients, suggesting a limited number of antigens or epitopes commonly recognized and (iii)A. actinomycetemcomitanslikely lacks superantigenic characteristics. These results suggestA. actinomycetemcomitans‐associated human CD4+T cell repertoire established in HuPBL‐NOD/SCID mice provides a useful approach to study specific aspects of immune–parasite interactions in the periodontium.