Time-Lapse, in Situ Imaging of Ice Crystal Growth Using Confocal Microscopy.
Time-Lapse, in Situ Imaging of Ice Crystal Growth Using Confocal Microscopy.
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DOI：
10.1021/acsomega.6b00217
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发表时间：
2016-11-30
期刊：
影响因子：
4.1
通讯作者：
Deville S
中科院分区：
文献类型：
--
作者：
Marcellini M;Noirjean C;Dedovets D;Maria J;Deville S
当水溶液被冷却到冰点以下时，冰晶就会成核和生长。冰晶的生长速度和形态取决于许多参数，例如冰生长的温度、融化温度以及溶质与生长晶体的相互作用。可能出现三种类型的形态：树枝状，细胞状（或指状），或多面平衡形式。理解和控制形成的形态类型在从生物学到生物物理学和材料科学的多个领域中是必不可少的。然而，由于实验技术，在不引入伪影的情况下原位获得三维观察结果是具有挑战性的。在这里，我们展示了如何使用激光扫描共聚焦显微镜实时跟踪醋酸锆溶液中光滑和多面冰晶的生长。可以进行定性和定量观察。特别是，我们可以精确地测量晶体的横向生长速度，否则很难获得的措施。这样的观察应该可以帮助我们了解控制各种系统中冰晶生长的参数的影响。
Ice crystals nucleate and grow when a water solution is cooled below its freezing point. The growth velocities and morphologies of the ice crystals depend on many parameters, such as the temperature of ice growth, the melting temperature, and the interactions of solutes with the growing crystals. Three types of morphologies may appear: dendritic, cellular (or fingerlike), or the faceted equilibrium form. Understanding and controlling which type of morphology is formed is essential in several domains, from biology to geophysics and materials science. Obtaining, in situ, three dimensional observations without introducing artifacts due to the experimental technique is nevertheless challenging. Here we show how we can use laser scanning confocal microscopy to follow in real-time the growth of smoothed and faceted ice crystals in zirconium acetate solutions. Both qualitative and quantitative observations can be made. In particular, we can precisely measure the lateral growth velocity of the crystals, a measure otherwise difficult to obtain. Such observations should help us understand the influence of the parameters that control the growth of ice crystals in various systems.