Lymphoma cell line (FL-18) and Epstein-Barr virus-carrying cell line (FL-18-EB) obtained from a patient with follicular lymphoma: monoclonal derivation and different properties.
Lymphoma cell line (FL-18) and Epstein-Barr virus-carrying cell line (FL-18-EB) obtained from a patient with follicular lymphoma: monoclonal derivation and different properties.
复制标题
DOI：
10.1182/blood.v70.5.1619.1619
复制
发表时间：
1987-11
期刊：
影响因子：
20.3
通讯作者：
S. Doi;H. Ohno;E. Tatsumi;Y. Arita;H. Kamesaki;S. Fukuhara;M. Nishikori;H. Miwa;K. Kita;M. Hatanaka
中科院分区：
文献类型：
--
作者：
S. Doi;H. Ohno;E. Tatsumi;Y. Arita;H. Kamesaki;S. Fukuhara;M. Nishikori;H. Miwa;K. Kita;M. Hatanaka
从滤泡小裂细胞淋巴瘤患者的胸腔积液中建立了一种新的细胞系FL-18。同时，从感染EBV的胸膜积液细胞培养物中建立EBV核抗原（EBNA）阳性细胞株FL-18-EB。这两种细胞系具有相同的单克隆表面免疫球蛋白（IgG κ pa），它们与新鲜胸腔积液细胞具有相同的核型，其中在染色体14和18的长臂之间检测到互惠易位[t(14;18)(q32;q21)]。免疫球蛋白重链基因（JH）和kappa轻链基因（J kappa）在两种细胞系中重排构型相同；然而，发现了一些形态和表型差异。FL-18- eb细胞在培养初期形态与正常B细胞来源的常见ena阳性淋巴母细胞样细胞系相似，比FL-18细胞大，含有多核巨细胞。FL-18细胞缺乏细胞质免疫球蛋白，对常见急性淋巴细胞白血病抗原（CALLA）呈阳性，而FL-18- eb细胞具有细胞质免疫球蛋白，对CALLA呈阴性。因此，FL-18- eb的表型似乎是eb病毒感染在B细胞分化途径中转向比FL-18更成熟阶段的结果。无EBV和感染EBV的肿瘤克隆细胞系的配对可用性在考虑EBV感染肿瘤B细胞和由此产生的表型变化方面是独特和有价值的。
A novel cell line, FL-18, was established from the pleural effusion of a patient with follicular small cleaved cell lymphoma. At the same time, an Epstein-Barr virus (EBV) nuclear antigen (EBNA)-positive cell line, FL-18-EB, was established from the EBV-infected culture of the same pleural effusion cells. Both cell lines had the same monoclonal surface immunoglobulin (IgG kappa), and they had the same karyotype as that of the fresh pleural effusion cells in which a reciprocal translocation between the long arm of chromosomes 14 and 18 [t(14;18)(q32;q21)] was detected. Gene rearrangement analysis of immunoglobulin heavy-chain gene (JH) and kappa light-chain gene (J kappa) showed the same rearranged configuration in the two cell lines; however, some morphological and phenotypic differences were found. The FL-18-EB cells, which were morphologically similar to common EBNA-positive lymphoblastoid cell lines of normal B cell origin at the initial phase of culture, were larger than the FL-18 cells and contained multinucleated giant cells. The FL-18 cells lacked cytoplasmic immunoglobulin and were positive for common acute lymphoblastic leukemia antigen (CALLA), whereas the FL-18-EB cells had cytoplasmic immunoglobulin and were negative for CALLA. Thus, the phenotype of FL-18-EB seems to be a result of a shift by EBV infection to a more mature stage in the B cell differentiation pathway than that of FL-18. The paired availability of EBV-free and EBV-infected cell lines of a neoplastic clone is unique and valuable in considering EBV infectibility of neoplastic B cells and resultant phenotypic changes.