Methods for Markerless Gene Deletion and Plasmid-Based Expression in Sulfolobus acidocaldarius.
Methods for Markerless Gene Deletion and Plasmid-Based Expression in Sulfolobus acidocaldarius.
复制标题
酸热硫化叶菌无标记基因删除和基于质粒的表达方法
DOI：
10.1007/978-1-0716-2445-6_8
复制
发表时间：
2022
影响因子：
--
通讯作者：
van Wolferen
中科院分区：
文献类型：
--
作者：
Recalde A;Albers SV;van Wolferen
功能良好的遗传系统对于研究体内基因功能非常重要，需要转化方法、载体系统和选择系统。酸热硫化叶菌是一种在 75 °C 和 pH 3 条件下生长最佳的穹窿模型生物。这些极端的生长条件给遗传系统的开发带来了一些困难。经过不断的努力，已经为硫化叶菌目的不同物种开发了通用的遗传工具。在本章中，我们描述了 S 中可用遗传工具的方法。酸热菌包括（1）转化方法，（2）产生无标记缺失突变体的弹出/弹出策略和（3）基于质粒的表达系统。
A well-functioning genetic system, which is important for studying gene functions in vivo, requires a transformation method, a vector system and a selection system.Sulfolobus acidocaldariusis a crenarchaeal model organism that grows optimally at 75 °C and a pH of 3. These extreme growth conditions cause some difficulties in developing a genetic system. With continuous efforts, versatile genetic tools have been developed for different species from the order of Sulfolobales. In this chapter, we describe the methods for the available genetic tools inS. acidocaldariusincluding a (1) transformation method, (2) pop in/pop out strategy to generate markerless deletion mutants and (3) a plasmid-based expression system.